- PN: B111398
45x Ab-conjugated beads (S4P10 - human IL-15 Ab-bead).PN: B111398A. One vial containing 100 µL of anti-human IL-15 conjugated to AimPlex Bead S4P10.
25x Biotin-detection Ab (human IL-15 Biotin-dAb).PN: B111398B. One vial containing 100 µL of biotinylated anti-human IL-15.
Lyophilized Standard Mix-Human Group 3 Panel B, 12-Plex. PN: HG3012B. One vial containing lyophilized recombinant human IL-9, IL-15, IL-16, IL-17C, IL-23p19, IL-24, IL-27, IL-31, IL-32, IL-34, OSM, and SCF. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
STORAGE: 2-8 C in the dark.
IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
APPLICATION: Optimal antibody pair and antigen standard for assaying human IL-15. Can be multiplexed with other analytes in Human Group 3. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 5 pg/mL
LLOQ: < 10 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Human Group 3: Negligible
Sample volume: 15 µL/test
Interleukin 15 (IL-15) is a cytokine that binds to and signals through a complex composed of IL-2/IL-15 receptor beta chain (CD122) and the common gamma chain (gamma-C, CD132). IL-15 is secreted by mononuclear phagocytes (and some other cells) following infection by viruses. IL-15 regulates T and NK cell activation and proliferation. Survival signals that maintain memory T cells in the absence of antigen are provided by IL-15. This cytokine is also implicated in NK cell development. In rodent lymphocytes, IL-15 prevents apoptosis by inducing an apoptosis inhibitor, BCL2L1/BCL-x(L). In humans with celiac disease IL-15 similarly suppresses apoptosis in T-lymphocytes by inducing Bcl-2 and/or Bcl-xL.
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