45x Ab-conjugated beads (S5P9 - human IL-17A Ab-bead). PN: A111146A. One vial containing 100 µL of anti-human IL-17A conjugated to AimPlex Bead S5P9.
25x Biotin-detection Ab (human IL-17A Biotin-dAb). PN: A111146B. One vial containing 100 µL of biotinylated anti-human IL-17A.
Lyophilized Standard Mix-Human Group 1 Panel A, 7-Plex. PN: HG10007. One vial containing lyophilized recombinant IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, and TNFα. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
STORAGE: 2-8 C in the dark.
IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
APPLICATION: Optimal antibody pair and antigen standard for assaying human IL-17A. Can be multiplexed with other analytes in Human Group 1. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 1 pg/mL
LLOQ: < 3 pg/mL
ULOQ: > 1,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Human Group 2: Negligible
Sample volume: 15 µL/test
Interleukin-17A (also known as CTLA8) is a pro-inflammatory cytokine produced by activated T cells. IL17A regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this IL17A are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. Diseases associated with IL17A include duodenal disease and paranasal sinus disease.
1. PDB: 1JPY; Hymowitz SG, Filvaroff EH, Yin JP, Lee J, Cai L, Risser P, Maruoka M, Mao W, Foster J, Kelley RF, Pan G, Gurney AL, de Vos AM, Starovasnik MA (October 2001). "IL-17s adopt a cystine knot fold: structure and activity of a novel cytokine, IL-17F, and implications for receptor binding". EMBO J. 20 (19): 5332–41. doi:10.1093/emboj/20.19.5332. PMC 125646. PMID 11574464.
2. Rouvier E, Luciani MF, Mattéi MG, Denizot F, Golstein P (Jun 1993). "CTLA-8, cloned from an activated T cell, bearing AU-rich messenger RNA instability sequences, and homologous to a herpesvirus saimiri gene". Journal of Immunology 150 (12): 5445–56. PMID 8390535.
3. Kuby, Janis; Kindt, Thomas J.; Goldsby, Richard A.; Osborne, Barbara A. (2007). Kuby immunology. San Francisco: W.H. Freeman. p. 396. ISBN 1-4292-0211-4.
4. Chiricozzi, A; Guttman-Yassky, E; Suárez-Fariñas, M; Nograles, K. E.; Tian, S; Cardinale, I; Chimenti, S; Krueger, J. G. (March 2011). "Integrative responses to IL-17 and TNF-α in human keratinocytes account for key inflammatory pathogenic circuits in psoriasis". J. Invest. Dermatol. 131 (3): 677–87. doi:10.1038/jid.2010.340. PMID 21085185.