• PN: B111402

COMPONENTS

45x Ab-conjugated beads (S5P6 - human 17C Ab-bead).PN: B111402A. One vial containing 100 µL of anti-human IL-17C conjugated to AimPlex Bead S5P6

25x Biotin-detection Ab (human IL-17C Biotin-dAb).PN: B111402B. One vial containing 100 µL of biotinylated anti-human IL-17C.

Lyophilized Standard Mix-Human Group 3 Panel B, 12-Plex. PN: HG3012B. One vial containing lyophilized recombinant human IL-9, IL-15, IL-16, IL-17C, IL-23p19, IL-24, IL-27, IL-31, IL-32, IL-34, OSM, and SCF.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human IL-17C.  Can be multiplexed with other analytes in Human Group 3.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 5 pg/mL

Quantitation range:

LLOQ: < 10 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 3: Negligible

Sample volume: 15 µL/test

Description:

Interleukin 17C (IL-17C) is a cytokine reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine is restricted to activated T cells. IL-17C plays a crucial role in innate immunity of the epithelium, including to intestinal bacterial pathogens, in an autocrine manner.  It stimulates the production of antibacterial peptides and pro-inflammatory molecules for host defense by signaling through the NF-kappa-B and MAPK pathways. It acts synergistically with IL22 in inducing the expression of antibacterial peptides, including S100A8, S100A9, REG3A and REG3G. Depending on the type of insult, may have both protective and pathogenic properties, either by maintaining epithelial homeostasis after an inflammatory challenge or by promoting inflammatory phenotype. Enhanced IL17C/IL17RE signaling may also lead to greater susceptibility to autoimmune diseases.

References:

1.      Interleukin-21 enhances NK cell activation in response to antibody-coated targets. Roda, J.M., Parihar, R., Lehman, A., Mani, A., Tridandapani, S., Carson, W.E. J. Immunol. (2006) [Pubmed]

2.      New IL-17 family members promote Th1 or Th2 responses in the lung: in vivo function of the novel cytokine IL-25. Hurst, S.D., Muchamuel, T., Gorman, D.M., Gilbert, J.M., Clifford, T., Kwan, S., Menon, S., Seymour, B., Jackson, C., Kung, T.T., Brieland, J.K., Zurawski, S.M., Chapman, R.W., Zurawski, G., Coffman, R.L. J. Immunol. (2002) [Pubmed]

3.      Autoimmunity during lymphopenia: a two-hit model. Krupica, T., Fry, T.J., Mackall, C.L. Clin. Immunol. (2006) [Pubmed]

4.      Cloning and characterization of IL-17B and IL-17C, two new members of the IL-17 cytokine family. Li, H., Chen, J., Huang, A., Stinson, J., Heldens, S., Foster, J., Dowd, P., Gurney, A.L., Wood, W.I. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]