• PN: B111426

COMPONENTS

45x Ab-conjugated beads (S5P8 - Human IL-18 Ab-bead). PN: B111426A. One vial containing 100 µL of anti-human IL-18 conjugated to AimPlex Bead S5P8.

25x Biotin-detection Ab (Human IL-18 Biotin-dAb). PN: B11426B. One vial containing 100 µL of biotinylated anti- Human IL-18.

Lyophilized Standard Mix - Human IL-18. PN: B11426S. One vial containing lyophilized IL-18.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human IL-18. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 5 pg/mL

Quantitation range:

LLOQ: <10 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Sample volume: 15 µL/test

Description:

IL-18 is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-γ (IFN-γ) or type II interferon that plays an important role in activating the macrophages or other cells. IL-18 is also able to induce severe inflammatory reactions, which suggests its role in certain inflammatory disorders.

References:

1.    Biet F, Locht C, Kremer L (2002). "Immunoregulatory functions of interleukin 18 and its role in defense against bacterial pathogens". J. Mol. Med. 80 (3): 147–62. doi:10.1007/s00109-001-0307-1. PMID 11894141.

2.      Nakanishi K (2002). "[Regulation of Th1 and Th2 immune responses by IL-18]". Kekkaku. 77 (2): 87–93. PMID 11905033.

3.      Reddy P, Ferrara JL (2003). "Role of interleukin-18 in acute graft-vs-host disease". J. Lab. Clin. Med. 141 (6): 365–71. doi:10.1016/S0022-2143(03)00028-3. PMID 12819633.

4.      Kanai T, Uraushihara K, Totsuka T, et al. (2003). "Macrophage-derived IL-18 targeting for the treatment of Crohn's disease". Current drug targets. Inflammation and allergy. 2 (2): 131–6. doi:10.2174/1568010033484250. PMID 14561165.