PN: A111128

COMPONENTS

45x Ab-conjugated beads (S4P5 - human IL-1β Ab-bead). PN: A111128A. One vial containing 100 µL of IL-1β conjugated to AimPlex Bead S4P5.

25x Biotin-detection Ab (human IL-1β Biotin-dAb). PN: A111122B. One vial containing 100 µL of biotinylated anti-human IL-1β.

Lyophilized Standard Mix-Human Group 1 Panel B. PN: HG10011. One vial containing lyophilized recombinant Eotaxin, IL-1β, IL-1RA, IL-8, IL-12p70, IL-22, IP-10, MCP-1, MCP-3, and RANTES.   Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits. 

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human IL-1β.  Can be multiplexed with other analytes in Human Group 1.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 5 pg/mL

Quantitation range:

LLOQ: < 10 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 1: Negligible

Sample volume: 15 µL/test

Description:

Interleukin-1 beta (IL-1β or IL1B) also known as '"leukocytic pyrogen"', "'leukocytic endogenous mediator'", "'mononuclear cell factor'", "'lymphocyte activating factor'" and other names, is a cytokine protein. IL-1β is a member of the interleukin 1 family of cytokines and is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Increased production of IL-1B causes a number of different auto-inflammatory syndromes, most notably the monogenic conditions referred to as CAPS, due to mutations in the inflammasome receptor NLRP3 which triggers processing of IL-1B.

References:

1.   Auron PE, Webb AC, Rosenwasser LJ, Mucci SF, Rich A, Wolff SM, Dinarello CA (1984). "Nucleotide sequence of human monocyte interleukin 1 precursor cDNA". Proc. Natl. Acad. Sci. U.S.A. 81 (24): 7907–11. doi:10.1073/pnas.81.24.7907. PMC 392262. PMID 6083565.

2.  "Catabolin" is the name given by Jeremy Saklatvala for IL-1 alpha. March CJ, Mosley B, Larsen A, Cerretti DP, Braedt G, Price V, Gillis S, Henney CS, Kronheim SR, Grabstein K (1985). "Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs". Nature 315 (6021): 641–7. doi:10.1038/315641a0. PMID 2989698.

3.  Clark BD, Collins KL, Gandy MS, Webb AC, Auron PE (1986). "Genomic sequence for human prointerleukin 1 beta: possible evolution from a reverse transcribed prointerleukin 1 alpha gene". Nucleic Acids Res 14 (20): 7897–1914. doi:10.1093/nar/14.20.7897. PMID 3490654Masters SL, Simon A, Aksentijevich I, Kastner DL (2009). "Horror autoinflammaticus: the molecular pathophysiology of autoinflammatory disease (*)". Annu. Rev. Immunol. 27: 621–68. doi:10.1146/annurev.immunol.25.022106.141627. PMC 2996236. PMID 19302049.