• PN: A113281

COMPONENTS

45x Ab-conjugated beads (S5P8-human sIL-1RI ab-bead). PN: A113281A. One vial containing 100 µL of anti-human sIL-1RI conjugated to AimPlex Bead S5P8.

25x Biotin-detection (human sIL-1RI Biotin-dAb). PN: A113281B. One vial containing100 µL of biotinylated anti-human sIL-1RI.

Lyophilized Standard Mix-Human Group 9 Panel A, 8-Plex. PN: HG9008A. One vial containing lyophilized recombinant human sFas, sICAM-3, sL-selectin, sP-selectin, sIL-1RI, sVEGFR1, sCD40, and sNCAM-1.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Human IL-1RI/sCD121a. Can be multiplexed with other analytes in Human Group 9.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 20 pg/mL

Quantitation range:

LLOQ: < 50 pg/mL

ULOQ: > 10,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 1: Negligible

Sample volume: 15 µL/test

Description:

Interleukin 1 receptor, type I (IL1R1) also known as CD121a, is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein is a receptor for interleukin 1 alpha (IL1A), interleukin 1 beta (IL1B), and interleukin 1 receptor antagonist (IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses. This gene along with interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.

References:

1.      Reddy, S A; Huang J H; Liao W S (November 1997). "Phosphatidylinositol 3-kinase in interleukin 1 signaling. Physical interaction with the interleukin 1 receptor and requirement in NFkappaB and AP-1 activation". J. Biol. Chem. (UNITED STATES) 272 (46): 29167–73. doi:10.1074/jbc.272.46.29167. ISSN 0021-9258. PMID 9360994.

2.      Burns, K; Clatworthy J; Martin L; Martinon F; Plumpton C; Maschera B; Lewis A; Ray K; Tschopp J; Volpe F (June 2000). "Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor". Nat. Cell Biol. (ENGLAND) 2 (6): 346–51. doi:10.1038/35014038. ISSN 1465-7392. PMID 10854325.

3.      Muzio, M; Ni J; Feng P; Dixit V M (November 1997). "IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling". Science (UNITED STATES) 278 (5343): 1612–5. doi:10.1126/science.278.5343.1612. ISSN 0036-8075. PMID 9374458.

4.      Huang, J; Gao X; Li S; Cao Z (November 1997). "Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin 1 receptor accessory protein". Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 94 (24): 12829–32. doi:10.1073/pnas.94.24.12829. ISSN 0027-8424. PMC 24223. PMID 9371760.