• PN: B113284

COMPONENTS

45x Ab-conjugated beads (S5P5 - Human IL-1RII Ab-bead). PN: A113284A. One vial containing 100 µL of anti-Human IL-1RII conjugated to AimPlex Bead S5P5.

25x Biotin-detection Ab (Human IL-1RII Biotin-dAb). PN: A113284B. One vial containing 100 µL of biotinylated anti-Human IL-1RII.

Lyophilized Standard Mix-Human Group 6, Panel B, 12-Plex. PN: HG62013. One vial containing lyophilized recombinant Eotaxin-2, IGFBP-1, IGFBP-7, MCP-4, MDC, MIP-1δ, MIP-3α, MIP-3β, IL-6Rα, IL-1RII, Gp130, and TNFRII. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human IL-1RII. Can be multiplexed with other analytes in Human Group 6.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 10 pg/mL

Quantitation range:

LLOQ: < 20 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 6: Negligible

Sample volume: 15 µL/test

Description:

Insulin-like growth factor-binding protein 7 (IGFBP-7) is a protein required for the regulation of the availability of insulin-like growth factors (IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP-7 binds to IGF with high affinity. It also stimulates cell adhesion. The protein is implicated in some cancers. Diseases associated with IGFBP7 include retinal arterial macroaneurysm with supravalvular pulmonic stenosis and familial retinal arterial macroaneurysm.

References:

1.      "Elevated levels of shed type II IL-1 receptor in sepsis. Potential role for type II receptor in regulation of IL-1 responses." Giri J.G., Wells J., Dower S.K., McCall C.E., Guzman R.N., Slack J., Bird T.A., Shanebeck K., Grabstein K.H., Sims J.E., Alderson M.R.; J. Immunol. 153:5802-5809(1994) [PubMed] [Europe PMC]

2.      "The type II IL-1 receptor interacts with the IL-1 receptor accessory protein: a novel mechanism of regulation of IL-1 responsiveness." Lang D., Knop J., Wesche H., Raffetseder U., Kurrle R., Boraschi D., Martin M.U. J. Immunol. 161:6871-6877(1998) [PubMed] [Europe PMC]

3.      "The membrane form of the type II IL-1 receptor accounts for inhibitory function." Neumann D., Kollewe C., Martin M.U., Boraschi D. J. Immunol. 165:3350-3357(2000) [PubMed] [Europe PMC]

4.      "The soluble form of IL-1 receptor accessory protein enhances the ability of soluble type II IL-1 receptor to inhibit IL-1 action." Smith D.E., Hanna R., Friend D., Moore H., Chen H., Farese A.M., MacVittie T.J., Virca G.D., Sims J.E. Immunity 18:87-96(2003) [PubMed] [Europe PMC]

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