• PN: B111406

COMPONENTS

45x Ab-conjugated beads (S5P12 - human IL-24 Ab-bead).PN: B111406A. One vial containing 100 µL of anti-human IL-24 conjugated to AimPlex Bead S5P12.

25x Biotin-detection Ab (human IL-24 Biotin-dAb).PN: B111406B. One vial containing 100 µL of biotinylated anti-human IL-24.

Lyophilized Standard Mix-Human Group 3 Panel B, 12-Plex. PN: HG3012B. One vial containing lyophilized recombinant human IL-9, IL-15, IL-16, IL-17C, IL-23p19, IL-24, IL-27, IL-31, IL-32, IL-34, OSM, and SCF.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human IL-24.  Can be multiplexed with other analytes in Human Group 3.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 5 pg/mL

Quantitation range:

LLOQ: < 10 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 3: Negligible

Sample volume: 15 µL/test

Description:

Interleukin 24 (IL-24) is a cytokine belonging to the IL-10 family of cytokines that signals through two heterodimeric receptors: IL-20R1/IL-20R2 and IL-22R1/IL-20R2. This interleukin is also known as melanoma differentiation-associated 7 (MDA-7) due to its discovery as a tumor suppressing protein. IL-24 appears to control in cell survival and proliferation by inducing rapid activation of particular transcription factors called STAT1 and STAT3. This cytokine is predominantly released by activated monocytes, macro-phages and T helper 2 (Th2) cells and acts on non-hematopoietic tissues such as skin, lung and reproductive tissues. IL-24 performs important roles in wound healing, psoriasis and cancer. Several studies have shown that cell death occurs in cancer cells/cell lines following exposure to IL-24. The gene for IL-24 is located on chromosome 1 in humans. Diseases associated with IL24 include melanoma and pancreatic cancer.

References:

1.      Poindexter NJ, Walch ET, Chada S, Grimm EA (September 2005). "Cytokine induction of interleukin-24 in human peripheral blood mononuclear cells". J. Leukoc. Biol. 78 (3): 745–52. doi:10.1189/jlb.0205116. PMID 16000394.

2.      Wang M, Liang P (February 2005). "Interleukin-24 and its receptors". Immunology 114 (2): 166–70. doi:10.1111/j.1365-2567.2005.02094.x. PMC 1782067. PMID 15667561.

3.      Fisher PB, Gopalkrishnan RV, Chada S, Ramesh R, Grimm EA, Rosenfeld MR, Curiel DT, Dent P (2003). "mda-7/IL-24, a novel cancer selective apoptosis inducing cytokine gene: from the laboratory into the clinic". Cancer Biol. Ther. 2 (4 Suppl 1): S23–37. PMID 14508078.

4.      Sauane M, Lebedeva IV, Su ZZ, Choo HT, Randolph A, Valerie K, Dent P, Gopalkrishnan RV, Fisher PB (May 2004). "Melanoma differentiation associated gene-7/interleukin-24 promotes tumor cell-specific apoptosis through both secretory and nonsecretory pathways". Cancer Res. 64 (9): 2988–93. doi:10.1158/0008-5472.CAN-04-0200. PMID 15126330.