96 Tests PN: B111406

Components:

45x Ab-conjugated beads (S5P12 - human IL-24 Ab-bead). PN: B111406A. One vial containing 100 µL of anti-human IL-24 conjugated to AimPlex Bead S5P12.

25x Biotin-detection Ab (human IL-24 Biotin-dAb). PN: B111406B. One vial containing 100 µL of biotinylated anti-human IL-24.

Lyophilized Standard Mix-Human Group 3 Panel B, 12-Plex. PN: HG3012B. One vial containing lyophilized recombinant human IL-9, IL-15, IL-16, IL-17C, IL-23p19, IL-24, IL-27, IL-31, IL-32, IL-34, OSM, and SCF.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human IL-24.  Can be multiplexed with other analytes in Human Group 3.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 5 pg/mL

  • Quantitation range:

  • LLOQ: < 10 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 3: Negligible

  • Sample volume: 15 µL/test

Description:

IL-24 New:

Interleukin 24 (Accession Q13007) is a member of the IL-10 cytokine family. IL-24 is predominantly secreted by immune cells such as myeloid, lymphoid, and Th2 cells. In normal skin, IL-24 suppresses keratinocyte proliferation during wound healing, offers protection against diseases caused by bacteria such as salmonella typhimurium and mycobacterium tuberculosis, and is involved in autoimmune diseases. IL-24 has been classified as an anti-cancer gene due to its ability to induce cell death in cancerous cells with little effect on surrounding normal cells. This cytokine can induce apoptosis using intracellular and extracellular signaling mechanisms.

References:

  1. Poindexter NJ, Walch ET, Chada S, Grimm EA. Cytokine induction of interleukin-24 in human peripheral blood mononuclear cells. J Leukoc Biol. 2005; 78(3): 745-52. Doi: 10.1189/jb.0205116.

  2. Sauane M, Lebedeva IV, Su ZZ, Choo HT, Randolph A, Valerie K, Dent P, Gopalkrishnan RV, Fisher pB> Melanoma differentiation associated gene-7/interleukin-24 promotes tumomr cell specific apoptosis through both secretory and non-secretory pathways. Cancer Res. 2004; 64(9): 2988-93. Doi: 10.1158/0008-5472.CAN-04-0200.

  3. Whitaker EL, Filippov VA, Duerksen-Hughes PJ. Interleukin-24: mechanisms and therapeutic potential of an anti-cancer gene. Cytokine Growth Factor Rev. 2012; 23(6): 323-31. Doi: 10.1016/j.cytogfr.2012.08.004.