• PN: B111407

COMPONENTS

45x Ab-conjugated beads (S5P5- Human IL-25 Ab-bead). PN: B111407A. One vial containing 100 µL of anti-human IL-25 conjugated to AimPlex Bead S5P5.

25x Biotin-detection Ab (Human IL-25 Biotin-dAb). PN: B111407B. One vial containing    100 µL of biotinylated anti-Human IL-25.

Lyophilized Standard Mix - Human IL-25. PN: B111407S. One vial containing lyophilized IL-25.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Human IL-25/IL17E. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 10 pg/mL

Quantitation range:

LLOQ: < 20 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Sample volume: 15 µL/test

Description:

Interleukin-25 (IL-25), also known as interleukin-17E (IL-17E), is a cytokine that shares the sequence similarity with IL-17. This cytokine can induce NF-κB activation, and stimulate the production of IL-8. Both this cytokine and IL-17B are ligands for the cytokine receptor IL-17RB. Studies of the similar gene in mice suggested that this cytokine may be a proinflammatory cytokine favoring Th2-type immune response. IL-25 is a cytokine that belongs to the IL-17 cytokine family and is secreted by type 2 helper T cells (Th2) and mast cells. IL-25 induces the production of other cytokines, including IL-4, IL-5 and IL-13 in multiple tissues, which stimulate the expansion of eosinophils. This cytokine is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract. IL-25 can kill some types of breast cancer cells.

References:

1.      Lee J, Ho WH, Maruoka M, Corpuz RT, Baldwin DT, Foster JS, Goddard AD, Yansura DG, Vandlen RL, Wood WI, Gurney AL (Mar 2001). "IL-17E, a novel proinflammatory ligand for the IL-17 receptor homolog IL-17Rh1". J Biol Chem 276 (2): 1660–4. doi:10.1074/jbc.M008289200. PMID 11058597.

2.      Fort MM, Cheung J, Yen D, Li J, Zurawski SM, Lo S, Menon S, Clifford T, Hunte B, Lesley R, Muchamuel T, Hurst SD, Zurawski G, Leach MW, Gorman DM, Rennick DM (Dec 2001). "IL-25 induces IL-4, IL-5, and IL-13 and Th2-associated pathologies in vivo". Immunity 15 (6): 985–95. doi:10.1016/S1074-7613(01)00243-6. PMID 11754819.

3.      "Ikeda K, Nakajima H, Suzuki K, Kagami S, Hirose K, Suto A, Saito Y, Iwamoto I (May 2003). "Mast cells produce interleukin-25 upon Fc epsilon RI-mediated activation". Blood 101 (9): 3594–6. doi:10.1182/blood-2002-09-2817. PMID 12511410.

4.      Owyang AM, Zaph C, Wilson EH, Guild KJ, McClanahan T, Miller HR, Cua DJ, Goldschmidt M, Hunter CA, Kastelein RA, Artis D (April 2006). "Interleukin 25 regulates type 2 cytokine-dependent immunity and limits chronic inflammation in the gastrointestinal tract". J. Exp. Med. 203 (4): 843–9. doi:10.1084/jem.20051496. PMC 1800834. PMID 16606667.