• PN: B111167

COMPONENTS

45x Ab-conjugated beads (S4P7 - human IL-27 Ab-bead). PN: B111167A. One vial containing 100 µL of anti-human IL-27 conjugated to AimPlex Bead S4P7.

25x Biotin-detection Ab (human IL-27 Biotin-dAb). PN: B111167B. One vial containing 100 µL of biotinylated anti-human IL-27.

Lyophilized Standard Mix-Human Group 3 Panel B, 12-Plex. PN: HG3012B. One vial containing lyophilized recombinant human IL-9, IL-15, IL-16, IL-17C, IL-23p19, IL-24, IL-27, IL-31, IL-32, IL-34, OSM, and SCF.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human IL-27.  Can be multiplexed with other analytes in Human Group 3.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 5 pg/mL

Quantitation range:

LLOQ: < 10 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 3: Negligible

Sample volume: 15 µL/test

Description:

Interleukin 27 (IL27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits; Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-30). IL-27 is produced by antigen-presenting cells. IL-27 plays an important function in regulating the activity of B- and T-lymphocytes. The effects of IL-27 are eliciting by its interaction with a specific cell-surface receptor complex composed of two proteins known as IL27R and gp130. Diseases associated with IL27 include eosinophilic granulomatosis with polyangiitis and posterior uveitis.

References:

1.      Larousserie, F; Bardel, E; Pflanz, S; Arnulf, B; Lome-Maldonado, C; Hermine, O; Brégeaud, L; Perennec, M; et al. (April 2005). "Analysis of interleukin-27 (EBI3/p28) expression in Epstein-Barr virus- and human T-cell leukemia virus type 1-associated lymphomas: heterogeneous expression of EBI3 subunit by tumoral cells". The American Journal of Pathology 166 (4): 1217–28. doi:10.1016/S0002-9440(10)62340-1. PMC 1602381. PMID 15793300.

2.      Lucas S, Ghilardi N, Li J, de Sauvage FJ (December 2003). "IL-27 regulates IL-12 responsiveness of naive CD4+ T cells through Stat1-dependent and -independent mechanisms". Proceedings of the National Academy of Sciences of the United States of America 100 (25): 15047–52. doi:10.1073/pnas.2536517100. PMC 299900. PMID 14657353.

3.      Larousserie F, Charlot P, Bardel E, Froger J, Kastelein RA, Devergne O (May 2006). "Differential effects of IL-27 on human B cell subsets". Journal of Immunology (Baltimore, Md. : 1950) 176 (10): 5890–7. doi:10.4049/jimmunol.176.10.5890. PMID 16670296.

4.      Pflanz S, Hibbert L, Mattson J, Rosales R, Vaisberg E, Bazan JF, Phillips JH, McClanahan TK, de Waal Malefyt R, Kastelein RA (February 2004). "WSX-1 and glycoprotein 130 constitute a signal-transducing receptor for IL-27". Journal of immunology (Baltimore, Md. : 1950) 172 (4): 2225–31. doi:10.4049/jimmunol.172.4.2225. PMID 14764690.