96 Tests PN: B111417
45x Ab-conjugated beads (S4P12 - human IL-32 Ab-bead). PN: B111417A. One vial containing 100 µL of anti-human IL-32 conjugated to AimPlex Bead S4P12
25x Biotin-detection Ab (human IL-32 Biotin-dAb). PN: B111417B. One vial containing 100 µL of biotinylated anti-human IL-32.
Lyophilized Standard Mix-Human Group 3 Panel B, 12-Plex. PN: HG3012B. One vial containing lyophilized recombinant human IL-9, IL-15, IL-16, IL-17C, IL-23p19, IL-24, IL-27, IL-31, IL-32, IL-34, OSM, and SCF. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
Application: Optimal antibody pair and antigen standard for assaying human IL-32. Can be multiplexed with other analytes in Human Group 3. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
Storage: 2-8 C in the dark.
Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 10 pg/mL
LLOQ: < 20 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Human Group 3: Negligible
Sample volume: 15 µL/test
Interleukin-31 (IL-31) is a cytokine with a four-helix bundle structure, which is preferentially produced by type 2 helper T cells (Th2). The structure of IL-31 places it in the IL-6 family of cytokines. IL-31 signals via a receptor complex that is composed of IL-31 receptor A (IL-31RA) and Oncostatin M receptor subunits. These receptor subunits are expressed in activated monocytes and in unstimulated epithelial cells. IL-31 is believed to play a role in inflammation of the skin.
Kim SH, Han SY, Azam T, Yoon DY, Dinarello CA (January 2005). "Interleukin-32: a cytokine and inducer of TNFalpha". Immunity 22 (1): 131–42. doi:10.1016/j.immuni.2004.12.003. PMID 15664165.
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BMP signaling coordinates gene expression and cell migration during precardiac mesoderm development. (PMID: 19913008) Christiaen L. … Levine M.(Dev. Biol. 2010)