96 Tests PN: B111422
45x Ab-conjugated beads (S5P4 - human IL-34 Ab-bead). PN: B111422A. One vial containing 100 µL of anti-human IL-34 conjugated to AimPlex Bead S5P4.
25x Biotin-detection Ab (human IL-34 Biotin-dAb). PN: B111422B. One vial containing 100 µL of biotinylated anti-human IL-34.
Lyophilized Standard Mix-Human Group 3 Panel B, 12-Plex. PN: HG3012B. One vial containing lyophilized recombinant human IL-9, IL-15, IL-16, IL-17C, IL-23p19, IL-24, IL-27, IL-31, IL-32, IL-34, OSM, and SCF. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
Application: Optimal antibody pair and antigen standard for assaying human IL-34 Can be multiplexed with other analytes in Human Group 3. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
Storage: 2-8 C in the dark.
Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 5 pg/mL
LLOQ: < 10 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Human Group 3: Negligible
Sample volume: 15 µL/test
Interleukin-34 (IL-34) belongs to a group of cytokines called interleukins. It was originally identified in humans, by large scale screening of secreted proteins; chimpanzee, murine, rat and chicken IL-34 orthologs have also been found. IL-34 increases growth or survival of monocytes; it elicits its activity by binding the Colony stimulating factor 1 receptor. Messenger RNA (mRNA) expression of human IL-34 is most abundant in spleen but occurs in several other tissues: thymus, liver, small intestine, colon, prostate gland, lung, heart, brain, kidney, testes, and ovary. The discovery of IL-34 protein in the red pulp of the spleen suggests involvement in growth and development of myeloid cells, consistent with its activity on monocytes. IL-34 promotes the release of pro-inflammatory chemo-kines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption.
Lin H, Lee E, Hestir K, et al. (May 2008). "Discovery of a cytokine and its receptor by functional screening of the extracellular proteome". Science 320 (5877): 807–11. doi:10.1126/science.1154370. PMID 18467591.
Franzè E, et al. “Interleukin-34 Induces Cc-chemokine Ligand 20 in Gut Epithelial Cells.” J Crohns Colitis. 2016 Jan;10(1):87-94. doi: 10.1093/ecco-jcc/jjv181. Epub 2015 Oct 8.
Interleukin-34 sustains inflammatory pathways in the gut. (PMID: 25800277) FranzA" E. … Monteleone G.(Clin. Sci. 2015)