PN: A111173

COMPONENTS

45x Ab-conjugated beads (S5P4 - human IL-4 ab-bead). PN: A111173A. One vial containing 100 µL of anti-human IL-4 conjugated To AimPlex Bead S5P4.

25x Biotin-detection Ab (human IL-4 Biotin-dAb). PN: A111173B. One vial containing 100 µL of biotinylated anti-human IL-4

Lyophilized Standard Mix-Human Group 1 Panel A, 7-Plex. PN: HG10007. One vial containing lyophilized recombinant IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, and TNFα. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human IL-4.  Can be multiplexed with other analytes in Human Group 1.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 1 pg/mL

Quantitation range:

LLOQ: < 3 pg/mL

ULOQ: > 1,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 1: Negligible

Sample volume: 15 µL/test

Description:

Interleukin-4 (Accession P05112) is produced by T-cells, mast cells, and basophils. IFN-γ and IL-4 are mutually antagonistic in their functions of differentiation and antibody class distinctions. IL-4 stimulates B and T-cell proliferation and differentiation and B-cell class switching from IgM to IgG1 and IgE. IL-4 can cross through the blood-brain barrier and even plays a constructive role in memory and learning within the brain. IL-4 has been demonstrated to drive dedifferentiation, and metastasis in rhabdomyosarcoma. Along with other Th2 cytokines, it is associated with airway inflammation and allergic reactions.

References:

  1. Choi P, Reiser H. IL-4: role in disease and regulation of production. Clin. Exp. Immunol. 1998; 113(3): 317-319. Doi:10.1046/j.1365-2249.1998.00690.x

  2. Gadani SP, Cronk JC, Norris GT, Kipnis J. IL-4 in the brain: a cytokine to remember. J Immunol. 2012;189(9):4213–4219. doi:10.4049/jimmunol.1202246.

  3. Hershey GK, Friedrich MF, Esswein LA, Thomas ML, Chatila TA. The association of atopy with a gain-of-function mutation in the alpha subunit of the interleukin-4 receptor. N. Engl. J. Med. 1997; 337(24): 1720-5. Doi:10.1056.NEJM199712113372403.

  4. Hosoyama T, Aslam MI, Abraham J, Prajapai SI, Nishijo K, Michalek JE, Zarazabal LA, Nelon LD, Guttridge DC, Rubin BP, Keller C. IL-4 Drives Dedifferentiation, Mitogenesis and Metastasis in Rhabdomyosarcoma. Clin. Cancer Res. 2011; 17(9): 2757-2766. Doi:10.1158/1078-0432.CCR-10-3445.