96 Tests PN: A111179

32 Tests PN: A111179T

Components:

45x Ab-conjugated beads (S5P6 - human IL-6 ab-bead).

  • 96 Tests PN: A111179A. One vial containing 100 µL of anti-human IL-6 conjugated to AimPlex Bead S5P6

  • 32 Tests PN: A111179TA. One vial containing 35 µL of anti-human IL-6 conjugated to AimPlex Bead S5P6

25x Biotin-detection Ab (human IL-6 Biotin-dAb).

  • 96 Tests PN: A111179B. One vial containing 100 µL of biotinylated anti-human IL-6.

  • 32 Tests PN: A111179TB. One vial containing 35 µL of biotinylated anti-human IL-6.

Lyophilized Standard Mix-Human Group 1 Panel A, 7-Plex.

  • 96 & 32 Tests PN: HG10007. One vial containing lyophilized recombinant IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, and TNFα. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits

Application: Optimal antibody pair and antigen standard for assaying human IL-6.  Can be multiplexed with other analytes in Human Group 1.  To be used in conjunction with the AimPlex NR Basic Kit (96 Tests PN: P100001, 32 Tests PN: P100001T) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 5 pg/mL

  • Quantitation range:

  • LLOQ: < 10 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 1: Negligible

  • Sample volume: 15 µL/test

Description:

Interleukin-6 (Accession P05231) is a member of the interleukin cytokine family, though it displays some hormone-like properties that can affect many metabolic processes. IL-6 may act as a pro- or anti-inflammatory cytokine. It is primarily secreted by T-cells and macrophages to stimulate immune responses. It may be secreted during tissue damage or during bacterial infections. IL-6 is able to cross the blood-brain barrier. IL-6 supports the growth of B-cells and inhibits the proliferation of regulatory T-cells. It may also have the potential to improve anti-tumor activity in cancer immunotherapy.

References:

  1. Banks WA, Kastin AJ, Gutierrez EG. Penetration of interleukin-6 across the murine blood-brain barrier. Neurosci. Lett. 1994; 179 (1-2): 53-56. Doi: 10.1016/0304-3940(94)90933-4.

  2. Ferguson-Smith AC, Chen YF, Newman MS, May LT, Sehgal PB, Ruddle FH. Regional localization of the interferon-beta 2/B-cell stimulatory factor 2/hepatocyte stimulating factor gene to human chromosome. Genomics. 1988; 2(3): 203-208. Doi:10.1016/0888-7543(88)90003-1.

  3. Jones SA, Takeuchi T, Aletaha D, Smolen J, Choy EH, McInnes I. Interleukin 6: The biology behind the therapy. BMJ. 2018; 2(1): Doi:10.1136/conmed-2018-000005.

  4. Lin G, Wang J, Lao X, Wang J, Li L, Li S, Zhang J, Dong Y, Chang AE, Li Q, Li S. Interleukin-6 inhibits regulatory T cells and improves the proliferation and cytotoxic activity of cytokine-induced killer cells. J Immunother. 2012; 35(4): 337-343. Doi: 10.1097/CJI.0b013e318255ada3.