96 Tests PN: B114314
Components:
45x Ab-conjugated beads (S5P11 - human Insulin Ab-bead). PN: B114314A. One vial containing 100 µL of anti-human Insulin conjugated to AimPlex Bead S5P11.
25x Biotin-detection Ab (human Insulin Biotin-dAb). PN: B114314B. One vial containing 100 µL of biotinylated anti-human Insulin.
Lyophilized Standard Mix-Human Group 4 Panel B, 10-Plex. PN: HG40010. One vial containing lyophilized recombinant human GASP-1, IFNβ, ANGPT-1, Insulin, Leptin, MIF, Resistin, Survivin, TGFα, and TPO. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
Application: Optimal antibody pair and antigen standard for assaying human Insulin. Can be multiplexed with other analytes in Human Group 4. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
Storage: 2-8 C in the dark.
Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
For Research Use Only. Not for use in diagnostic procedures.
Assay Specifications:
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 10 pg/mL
Quantitation range:
LLOQ: < 20 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Human Group 4: Negligible
Sample volume: 15 µL/test
Description:
Insulin (Accession P01308), a peptide hormone composed of 51 amino acids is produced in the pancreas by beta cells. It is considered the primary anabolic hormone as its production is stimulated by the introduction of glucose. Insulin, first synthesized as prepoinsulin, undergoes maturation by activation of carboxypeptidase E and prohormone convertases. Mature insulin then awaits exocytosis from pancreatic cells. Insulin is largely regulated by transcription factors PDX1, NeuroD1, and MafA. Along with IGF-I and IGF-II, Insulin produced in the brain has links to Alzheimer’s disease. Diseases associated with irregular insulin production include Type 1 and Type 2 diabetes, insulinoma, metabolic syndrome, and polycystic ovary syndrome.
References:
Voet D, Voet JG. Biochemistry. 2011. 4th Edition. New York: Wiley.
Bernardo AS, Hay CW, Docherty K. Pancreatic transcription factors and their role in the birth, life, and survival of the pancreatic beta cell. Mol Cell Endocrinol. 2008; 294(1-2): 1-9. Doi:10.1016/j.mce.2008.07.006.
De la Monte SM, Wands JR. Review of insulin and insulin-like growth factor expression, signaling, and malfunction in the central nervous system: Relevance to Alzheimer’s disease. JAD. 2005; 7(1) 45-61. Doi: 10.3233/JAD-2005-7106.