96 Tests PN: B111326
45x Ab-conjugated beads (S5P7- human KGF Ab-bead). PN: B111326A. One vial containing 100 µL of anti-human KGF conjugated to AimPlex Bead S5P7.
25x Biotin-detection Ab (human KGF Biotin-dAb). PN: B111326B. One vial containing 100 µL of biotinylated anti-human KGF.
Lyophilized Standard Mix-Human Group 10, Panel A, 9-Plex. PN: HG1009A. One vial containing lyophilized recombinant BTC, CNTF, FGF-4, Follistatin, IL-19, KGF, PDGF-AA, PDGF-BB, and PIGF-1. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
Application: Optimal antibody pair and antigen standard for assaying human Human FGF-7/KGF. Can be multiplexed with other analytes in Human Group 10. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
Storage: 2-8 C in the dark.
Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 10 pg/mL
LLOQ: < 20 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Human Group 10: Negligible
Sample volume: 15 µL/test
Keratinocyte Growth Factor (KGF), also known as FGF7, is a growth factor present in the epithelialization-phase of wound healing. In this phase, keratinocytes are covering the wound, forming the epithelium. KGF is a small signaling molecule that binds to fibroblast growth factor receptor 2b (FGFR2b). For signaling to occur, a dimer is required between two FGF: FGFR complexes that is linked together by a molecule of heparin. There are 23 known FGFs, and 4 FGF receptors. FGF: FGFR binding is complex and regulated by a variety of mechanisms in a tissue specific manner.
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