• PN: B111326

COMPONENTS

45x Ab-conjugated beads (S5P7- human KGF Ab-bead). PN: B111326A. One vial containing 100 µL of anti-human KGF conjugated to AimPlex Bead S5P7.

25x Biotin-detection Ab (human KGF Biotin-dAb). PN: B111326B. One vial containing 100 µL of biotinylated anti-human KGF.

Lyophilized Standard Mix-Human Group 10, Panel A, 9-Plex. PN: HG1009A. One vial containing lyophilized recombinant BTC, CNTF, FGF-4, Follistatin, IL-19, KGF, PDGF-AA, PDGF-BB, and PIGF-1.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Human FGF-7/KGF. Can be multiplexed with other analytes in Human Group 10.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 10 pg/mL

Quantitation range:

LLOQ: < 20 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 10: Negligible

Sample volume: 15 µL/test

Description:

The Keratinocyte Growth Factor (KGF), also known as FGF7, is a growth factor present in the epithelialization-phase of wound healing. In this phase, keratinocytes are covering the wound, forming the epithelium. KGF is a small signaling molecule that binds to fibroblast growth factor receptor 2b (FGFR2b). For signaling to occur, a dimer is required between two FGF: FGFR complexes that is linked together by a molecule of heparin. There are 23 known FGFs, and 4 FGF receptors. FGF: FGFR binding is complex and regulated by a variety of mechanisms in a tissue specific manner.

References:

1.      Rotolo S, Ceccarelli S, Romano F, Frati L, Marchese C, Angeloni A (2008). Maas, Stefan, ed. "Silencing of Keratinocyte Growth Factor Receptor Restores 5-Fluorouracil and Tamoxifen Efficacy on Responsive Cancer Cells". PLoS ONE 3 (6): e2528. doi:10.1371/journal.pone.0002528. PMC 2424182. PMID 18575591. open access publication - free to read

2.      Mattei MG, deLapeyrière O, Bresnick J, Dickson C, Birnbaum D, Mason I (Mar 1995). "Mouse Fgf7 (fibroblast growth factor 7) and Fgf8 (fibroblast growth factor 8) genes map to chromosomes 2 and 19 respectively". Mammalian Genome 6 (3): 196–7. doi:10.1007/BF00293012. PMID 7749227.

3.      Kelley MJ, Pech M, Seuanez HN, Rubin JS, O'Brien SJ, Aaronson SA (Oct 1992). "Emergence of the keratinocyte growth factor multigene family during the great ape radiation". Proceedings of the National Academy of Sciences of the United States of America 89 (19): 9287–91. doi:10.1073/pnas.89.19.9287. PMC 50111. PMID 1409637.

4.      Mongiat M, Taylor K, Otto J, Aho S, Uitto J, Whitelock JM, Iozzo RV (Mar 2000). "The protein core of the proteoglycan perlecan binds specifically to fibroblast growth factor-7". The Journal of Biological Chemistry 275 (10): 7095–100. doi:10.1074/jbc.275.10.7095. PMID 10702276.