• PN: A113272

COMPONENTS

45x Ab-conjugated beads (S5P6 - human sL-selectin Ab-bead). PN: A113272A. One vial containing 100 µL of anti-human sL-selectin conjugated to AimPlex Bead S5P6.

25x Biotin-detection (human sL-selectin Biotin-dAb). PN: A113272B. One vial containing 100 µL of biotinylated anti-human sL-selectin.

Lyophilized Standard Mix-Human Group 9 Panel A, 8-Plex. PN: HG9008A. One vial containing lyophilized recombinant human sFas, sICAM-3, sL-selectin, sP-selectin, sIL-1RI, sVEGFR1, sCD40, and sNCAM-1.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Human sL-selectin/LECAM­1/sCD62L. Can be multiplexed with other analytes in Human Group 9.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 50 pg/mL

Quantitation range:

LLOQ: < 100 pg/mL

ULOQ: > 50,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 9: Negligible

Sample volume: 15 µL/test

Description:

L-selectin, also known as CD62L, is a cell adhesion molecule found on lymphocytes and the preimplantation embryo. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups.  L-selectin is a cell surface component that is a member of a family of adhesion/homing receptors that play important roles in lymphocyte-endothelial cell interactions. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues. Diseases associated with L-selectin include cryptococcosis and arthus reaction.

References:

1.      Gallatin, W. M., I. L. Weissman, et al. (1983). "A cell-surface molecule involved in organ-specific homing of lymphocytes." Nature 304(5921): 30-4.

2.      Ryan US, Worthington RE (1992). "Cell-cell contact mechanisms". Curr. Opin. Immunol. 4 (1): 33–7. doi:10.1016/0952-7915(92)90120-4. PMID 1375831.

3.      Nicholson IC (2003). "CD62L (L-selectin)". J. Biol. Regul. Homeost. Agents 16 (2): 144–6. PMID 12144128.

4.      Siegelman, M. H., I. C. Cheng, et al. (1990). "The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding." Cell 61(4): 611-22.