• PN: B112492

COMPONENTS

45x Ab-conjugated beads (S5P5 - human Lymphotactin Ab-bead). PN: B112492A. One vial containing 100 µL of anti-human Lymphotactin conjugated to AimPlex Bead S5P5.

25x Biotin-detection Ab (human Lymphotactin Biotin-dAb). PN: B112492B. One vial containing 100 µL of biotinylated anti-human Lymphotactin.

Lyophilized Standard Mix-Human Group 10, Panel B, 7-Plex. PN: HG1007B. One vial containing lyophilized recombinant Lymphotactin, MIA, Midkine, NOV, NRG1-beta1, sTACI, and sTRAIL. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Human Lymphotactin/XCL1/SCYC1. Can be multiplexed with other analytes in Human Group 10.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 10 pg/mL

Quantitation range:

LLOQ: < 20 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 10: Negligible

Sample volume: 15 µL/test

Description:

Chemokine (C motif) ligand (XCL1) is a small cytokine belonging to the XC chemokine family that is also known as Lymphotactin and small-inducible cytokine subfamily C, Member 1 (SCYC1). It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. Cellular sources for XCL1 include activated thymic and peripheral blood CD8+ T cells. This chemokine attracts T cells. In humans, XCL1 is closely related to another chemokine called XCL2, whose gene is found at the same locus on chromosome 1. XCL1 induces it chemotactic function by binding to a chemokine receptor called XCR1. Diseases associated with XCL1 include renal tuberculosis.

References:

1.      Kelner G, Kennedy J, Bacon K, Kleyensteuber S, Largaespada D, Jenkins N, Copeland N, Bazan J, Moore K, Schall T, Zlotnik A (1994). "Lymphotactin: a cytokine that represents a new class of chemokine". Science 266 (25): 1395–9. PMID 7973732.

2.      Kennedy J, Kelner G, Kleyensteuber S, Schall T, Weiss M, Yssel H, Schneider P, Cocks B, Bacon K, Zlotnik A (1995). "Molecular cloning and functional characterization of human lymphotactin". J Immunol 155 (1): 203–9. PMID 7602097.

3.      Yoshida T, Imai T, Takagi S, Nishimura M, Ishikawa I, Yaoi T, Yoshie O (1996). "Structure and expression of two highly related genes encoding SCM-1/human lymphotactin". FEBS Lett 395 (1): 82–8. doi:10.1016/0014-5793(96)01004-6. PMID 8849694.

4.      Yoshida T, Imai T, Kakizaki M, Nishimura M, Takagi S, Yoshie O (1998). "Identification of single C motif-1/lymphotactin receptor XCR1". J Biol Chem 273 (26): 16551–4. doi:10.1074/jbc.273.26.16551. PMID 9632725.