PN: A112233

COMPONENTS

45x Ab-conjugated beads (S5P1 - human MCP-1 ab-bead). PN: A112233A. One vial containing 100 µL of anti-human MCP-1 conjugated to AimPlex Bead S5P1.

25x Biotin-detection Ab (human MCP-1 Biotin-dAb). PN: A112233B. One vial containing 100 µL of biotinylated anti-human MCP-1.

Lyophilized Standard Mix-Human Group 1 Panel B. PN: HG10011. One vial containing lyophilized recombinant Eotaxin, IL-1β, IL-1RA, IL-8, IL-12p70, IL-22, IP-10, MCP-1, MCP-3, and RANTES. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits. 

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human CCL2/SCYA2/MCP-1.  Can be multiplexed with other analytes in Human Group 1.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 2 pg/mL

Quantitation range:

LLOQ: <5 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 1: Negligible

Sample volume: 15 µL/test

Description:

MCP-1 or Monocyte Chemoattractant Protein 1 (Accession P13500) is one of the most frequently studied cytokines. MCP-1 is a small cytokine of the CC subfamily secreted primarily by monocytes, though it is also secreted in microcytic, astrocytic, mesangial, endothelial, epithelial cells and even adipokines. MCP-1 regulates migration of monocytes, NK cells, and memory T-cells. Elevated MCP-1 may change adipocyte function. Insulin-stimulated glucose uptake and expression of various adipogenic genes have demonstrated to be affected by elevated levels MCP-1. Changes in glucose-uptake has correlations to metabolic and various central nervous system diseases. MCP-1 has been shown to be a potential intervention point for treatment of sclerosis, atherosclerosis, insulin-resistant diabetes, and rheumatoid arthritis.

References:

  1. Carr MW, Roth SJ, Luther E, Rose SS, Springer TA. Monocyte chemoattractant protein 1 acts as a T-lymphocyte chemoattractant. Proceedings of the National Academy of Sciences of the United States of America 91 (9): 3652–6. doi:10.1073/pnas.91.9.3652. PMC 43639. PMID 8170963.

  2. Deshmane SL, Kremlev S, Amini S, Sawaya BE. Monocyte chemoattractant protein-1 (MCP-1): an overview. J Interferon Cytokine Res. 2009;29(6):313–326. doi:10.1089/jir.2008.0027.

  3. Kim MS, Day CJ, Morrison NA. MCP-1 is induced by receptor activator of nuclear factor-{kappa}B ligand, promotes human osteoclast fusion, and rescues granulocyte macrophage colony-stimulating factor suppression of osteoclast formation. The Journal of Biological Chemistry 280 (16): 16163–9. doi:10.1074/jbc.M412713200. PMID 15722361.

  4. Xu LL, Warren MK, Rose WL, Gong, W, Wang JM. Human recombinant monocyte chemotactic protein and other C-C chemokines bind and induce directional migration of dendritic cells in vitro. Journal of Leukocyte Biology 60 (3): 365–71. PMID 8830793.