• PN: B112500

COMPONENTS

45x Ab-conjugated beads (S4P4 - Human MCP-4 Ab-bead). PN: B112500A. One vial containing 100 µL of anti-Human MCP-4 conjugated to AimPlex Bead S4P4.

25x Biotin-detection Ab (Human MCP-4 Biotin-dAb). PN: B112500B. One vial containing 100 µL of biotinylated anti-Human MCP-4.

Lyophilized Standard Mix-Human Group 6, Panel B, 12-Plex. PN: HG62013. One vial containing lyophilized recombinant Eotaxin-2, IGFBP-1, IGFBP-7, MCP-4, MDC, MIP-1δ, MIP-3α, MIP-3β, IL-6Rα, IL-1RII, Gp130, and TNFRII. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying humanCCL13/SCYA13/MCP-4. Can be multiplexed with other analytes in Human Group 6.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 10 pg/mL

Quantitation range:

LLOQ: < 20 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 6: Negligible

Sample volume: 15 µL/test

Description:

Chemokine (C-C motif) ligand 13 (CCL13) that was previously called monocyte-specific chemokine 4 (MCP4) and small inducible cytokine A13 (SYCA13) is a small cytokine belonging to the CC chemokine family. CCL13 induces chemotaxis in monocytes, eosinophils, T lymphocytes, and basophils by binding cell surface G-protein linked chemokine receptors such as CCR2, CCR3 and CCR5. Activity of this chemokine has been implicated in allergic reactions such as asthma. CCL13 can be induced by the inflammatory cytokines interleukin-1 and TNF-α. Diseases associated with CCL13 include far eastern spotted fever and spotted fever.

References:

1.      Garcia-Zepeda EA, et al.. Human monocyte chemoattractant protein (MCP)-4 is a novel CC chemokine with activities on monocytes, eosinophils, and basophils induced in allergic and no allergic inflammation that signals through the CC chemokine receptors (CCR)-2 and -3. J Immunol. 1996;157:5613–5626

2.      Naruse et al., A YAC contig of the human CC chemokine genes clustered on chromosome 17q11.2. Genomics. 1996, 34(2):236-40.

3.      Blanpain et al., CCR5 binds multiple CC-chemokines: MCP-3 acts as a natural antagonist. Blood. 1999, 94:1899-905.

4.      Lamkhioued et al., Monocyte chemoattractant protein (MCP)-4 expression in the airways of patients with asthma. Induction in epithelial cells and mononuclear cells by proinflammatory cytokines. Am J Respir Crit Care Med. 2000, 162:723-32.

.