• PN: B111432

COMPONENTS

45x Ab-conjugated beads (S5P12- human Midkine Ab-bead). PN: B111432A. One vial containing 100 µL of anti-human Midkine conjugated to AimPlex Bead S5P12.

25x Biotin-detection Ab (human Midkine Biotin-dAb). PN: B111432B. One vial containing 100 µL of biotinylated anti-human Midkine.

Lyophilized Standard Mix-Human Group 10, Panel B, 7-Plex. PN: HG1007B. One vial containing lyophilized recombinant Lymphotactin, MIA, Midkine, NOV, NRG1-beta1, sTACI, and sTRAIL. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Human Midkine/MK1/NEGF-2. Can be multiplexed with other analytes in Human Group 10.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 10 pg/mL

Quantitation range:

LLOQ: < 20 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 10: Negligible

Sample volume: 15 µL/test

Description:

Midkine (MK or MDK) also known as neurite growth-promoting factor 2 (NEGF2) is a basic heparin-binding growth factor and forms a family with pleiotrophin (NEGF1, 46% homologous with MK).  It is a developmentally important retinoic acid-responsive gene product strongly induced during mid-gestation, hence the name midkine. Restricted mainly to certain tissues in the normal adult, it is strongly induced during oncogenesis, inflammation and tissue repair. MK is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. Diseases associated with MDK include mesomelic dysplasia, kantaputra type and periapical granuloma. MK appears to enhance the angiogenic and proliferative activities of cancer cells.  It is also involved in early fetal adrenal gland development. Midkine binds to cell-surface nucleolin as a low affinity receptor, which can inhibit HIV infection.

References:

1.      Kaname T, Kuwano A, Murano I, Uehara K, Muramatsu T, Kajii T (August 1993). "Midkine gene (MDK), a gene for prenatal differentiation and neuroregulation, maps to band 11p11.2 by fluorescence in situ hybridization". Genomics 17 (2): 514–5. doi:10.1006/geno.1993.1359. PMID 8406506.

2.      Muramatsu Takashi (1 September 2002). "Midkine and pleiotrophin: two related proteins involved in development, survival, inflammation and tumorigenesis". J. Biochem. (Tokyo) 132 (3): 359–71. doi:10.1093/oxfordjournals.jbchem.a003231. PMID 12204104.

3.      Kato M, Maeta H, Kato S, Shinozawa T, Terada T (October 2000). "Immunohistochemical and in situ hybridization analyses of midkine expression in thyroid papillary carcinoma". Mod. Pathol. 13 (10): 1060–5. doi:10.1038/modpathol.3880195. PMID 11048798.Miyakawa S, Fujiwara M, Nakamura Y, Matsui T, and Sakuma S. Aptamer against midkine and use thereof. United States Patent 8080649, 2011.