96 Tests PN: A112236
45x Ab-conjugated beads (S5P5 - human MIG Ab-bead). PN: A112236A. One vial containing 100 µL of anti-human MIGconjugated to AimPlex Bead S5P5.
25x Biotin-detection Ab (human MIG Biotin-dAb). PN: A112236B. One vial containing 100 µL of biotinylated anti-human MIG.
Lyophilized Standard Mix - Human MIG. PN: HMIGAG1. One vial containing lyophilized recombinant MIG.
Application: Optimal antibody pair and antigen standard for assaying human CXCL9/SCYB9/MIG. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
Storage: 2-8 C in the dark.
Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 5 pg/mL
LLOQ: <10 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Sample volume: 15 µL/test
CXCL9 (Chemokine C-X-C motif ligand 9) is a small cytokine belonging to the CXC chemokine family that is also known as small-inducible cytokine B9 (SCYB9) and Monokine induced by gamma interferon (MIG). CXCL9 is a T-cell chemoattractant, which is induced by IFN-γ. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4. CXCL9, CXCL10 and CXCL11 all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. Neutrophil collagenase/matrix metalloproteinase 8 (MMP-8) degrades CXCL9 and cleaves CXCL10 at two positions. Gelatinase B/matrix metalloproteinase 9 (MMP-9) degrades CXCL10 and cleaves CXCL9 at three different sites in its extended carboxy-terminal region. Diseases associated with CXCL9 include lymphomatoid granulomatosis and endotheliitis.
Lee HH, Farber JM (1996). "Localization of the gene for the human MIG cytokine on chromosome 4q21 adjacent to INP10 reveals a chemokine "mini-cluster"". Cytogenetics and Cell Genetics 74 (4): 255–8. doi:10.1159/000134428. PMID 8976378.
O'Donovan N, Galvin M, Morgan JG (1999). "Physical mapping of the CXC chemokine locus on human chromosome 4". Cytogenetics and Cell Genetics 84 (1-2): 39–42. doi:10.1159/000015209. PMID 10343098.
Tensen CP, Flier J, Van Der Raaij-Helmer EM, Sampat-Sardjoepersad S, Van Der Schors RC, Leurs R, Scheper RJ, Boorsma DM, Willemze R (May 1999). "Human IP-9: A keratinocyte-derived high affinity CXC-chemokine ligand for the IP-10/Mig receptor (CXCR3)". The Journal of Investigative Dermatology 112 (5): 716–22. doi:10.1046/j.1523-1747.1999.00581.x. PMID 10233762.
Lasagni L, Francalanci M, Annunziato F, Lazzeri E, Giannini S, Cosmi L, Sagrinati C, Mazzinghi B, Orlando C, Maggi E, Marra F, Romagnani S, Serio M, Romagnani P (Jun 2003). "An alternatively spliced variant of CXCR3 mediates the inhibition of endothelial cell growth induced by IP-10, Mig, and I-TAC, and acts as functional receptor for platelet factor 4". The Journal of Experimental Medicine 197 (11): 1537–49. doi:10.1084/jem.20021897. PMC 2193908. PMID 12782716.