96 Tests PN: B112506

Components:

45x Ab-conjugated beads (S5P7 - Human MIP-1δ Ab-bead). PN: B112506A. One vial containing 100 µL of anti-Human MIP-1δ conjugated to AimPlex Bead S5P7.

25x Biotin-detection Ab (Human MIP-1δ Biotin-dAb). PN: B112506B. One vial containing 100 µL of biotinylated anti-Human MIP-1δ.

Lyophilized Standard Mix-Human Group 6, Panel B, 12-Plex. PN: HG62013. One vial containing lyophilized recombinant Eotaxin-2, IGFBP-1, IGFBP-7, MCP-4, MDC, MIP-1δ, MIP-3α, MIP-3β, IL-6Rα, IL-1RII, Gp130, and TNFRII. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human CCL15/SCYA15/MIP-1δ. Can be multiplexed with other analytes in Human Group 6.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 10 pg/mL

  • Quantitation range:

  • LLOQ: < 20 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 6: Negligible

  • Sample volume: 15 µL/test

Description:

Chemokine (C-C motif) ligand 15 (CCL15) is a small cytokine belonging to the CC chemokine family that is also known as leukotactin-1, MIP-1 delta, MIP-5, Small Inducible Cytokine Subfamily A (Cys-Cys)15 (SCYA15) and HCC-2.  CCL15 is expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. It is chemotactic for neutrophils, monocytes, and lymphocytes and elicits its effects by binding to cell surface chemokine receptors like CCR1 and CCR3.  Diseases associated with CCL15 include pertussis.

References:

  1. Pardigol et al. HCC-2, a human chemokine: gene structure, expression pattern, and biological activity. Proc. Nat. Acad. Sci. 95: 6308-6313, 1998.

  2. Youn et al. Molecular cloning of leukotactin-1: a novel human beta-chemokine, a chemoattractant for neutrophils, monocytes, and lymphocytes, and a potent agonist at CC chemokine receptors 1 and 3. J. Immun. 159: 5201-5205, 1997.

  3. Coulin et al. Characterization of macrophage inflammatory protein-5/human CC cytokine-2, a member of the macrophage-inflammatory-protein family of chemokines. Europ. J. Biochem. 248: 507-515, 1997.

  4. Naruse et al. A YAC contig of the human CC chemokine genes clustered on chromosome 17q11.2. Genomics 34: 236-240, 1996.