- PN: B112509
45x Ab-conjugated beads (S4P2 - Human MIP-3α Ab-bead). PN: B112509A. One vial containing 100 µL of anti-Human MIP-3α conjugated to AimPlex Bead S4P2.
25x Biotin-detection Ab (Human MIP-3α Biotin-dAb). PN: B112509B. One vial containing 100 µL of biotinylated anti-Human MIP-3α.
Lyophilized Standard Mix-Human Group 6, Panel B, 12-Plex. PN: HG62013. One vial containing lyophilized recombinant Eotaxin-2, IGFBP-1, IGFBP-7, MCP-4, MDC, MIP-1δ, MIP-3α, MIP-3β, IL-6Rα, IL-1RII, Gp130, and TNFRII. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
STORAGE: 2-8 C in the dark.
IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
APPLICATION: Optimal antibody pair and antigen standard for assaying human CCL20/SCYA20/MIP-3α. Can be multiplexed with other analytes in Human Group 6. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 10 pg/mL
LLOQ: < 20 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Human Group 6: Negligible
Sample volume: 15 µL/test
Chemokine (C-C motif) ligand 20 (CCL20) or Small Inducible Cytokine Subfamily A (Cys-Cys) 20 (SCYA20), liver activation regulated chemokine (LARC) or Macrophage Inflammatory Protein-3 (MIP3A) is a small cytokine belonging to the CC chemokine family. It is strongly chemotactic for lymphocytes and weakly attracts neutrophils. CCL20 is implicated in the formation and function of mucosal lymphoid tissues via chemoattraction of lymphocytes and dendritic cells towards the epithelial cells surrounding these tissues. CCL20 elicits its effects on its target cells by binding and activating the chemokine receptor CCR6. Gene expression of CCL20 can be induced by microbial factors such as lipopolysaccharide, and inflammatory cytokines such as tumor necrosis factor and interferon-γ, and down-regulated by IL-10. CCL20 is expressed in several tissues with highest expression observed in peripheral blood lymphocytes, lymph nodes, liver, appendix, and fetal lung and lower levels in thymus, testis, prostate and gut. Diseases associated with CCL20 include hordeolum, smallpox and stromal keratitis.
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