• PN: B112513

COMPONENTS

45x Ab-conjugated beads (S4P11- Human MPIF-1 Ab-bead). PN: B112513A. One vial containing 100 µL of anti-Human MPIF-1 conjugated to AimPlex Bead S4P11.

25x Biotin-detection Ab (Human MPIF-1 Biotin-dAb). PN: B112513B. One vial containing 100 µL of biotinylated anti-Human MPIF-1.

Lyophilized Standard Mix-Human Group 7, Panel A, 8-Plex. PN: HG71008. One vial containing lyophilized recombinant Eotaxin-3, GCP-2, GROα, HCC-1, HCC-4, I-309, MCP-2, and MPIF-1. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human CCL23/SYCA23/MPIF-1. Can be multiplexed with other analytes in Human Group 7.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 10 pg/mL

Quantitation range:

LLOQ: < 20 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 7: Negligible

Sample volume: 15 µL/test

Description:

Chemokine (C-C motif) ligand 23 (CCL23) is a small cytokine belonging to the CC chemokine family that is also known as small inducible cytokine A23 (SYCA23),  Macrophage Inflammatory Protein 3 (MIP-3) and Myeloid Progenitor Inhibitory factor 1 (MPIF-1). CCL23 is predominantly expressed in lung and liver tissue, but is also found in bone marrow and placenta.  It is also expressed in some cell lines of myeloid origin. CCL23 is highly chemotactic for resting T cells and monocytes and slightly chemotactic for neutrophils. It has also been attributed to an inhibitory activity on hematopoietic progenitor cells. Diseases associated with CCL23 include sialadenitis.

References:

1.      Patel et al. Molecular and functional characterization of two novel human C-C chemokines as inhibitors of two distinct classes of myeloid progenitors. J. Exp. Med. 185: 1163-1172, 1997.

2.      Berahovich et al. Proteolytic Activation of Alternative CCR1 Ligands in Inflammation. J. Immunol. 174:7341-7351, 2005.

3.      CK beta 8/CCL23 induces cell migration via the Gi/Go protein/PLC/PKC delta/NF-kappa B and is involved in inflammatory responses. (PMID: 19951712) Kim J. … Ko J.(Life Sci. 2010).