96 Tests PN: B113383

Components:

45x Ab-conjugated beads (S5P7- human sB7-DC Ab-bead). PN: B113383A. One vial containing 100 µL of anti-human sB7-DC conjugated to AimPlex Bead S5P7.

25x Biotin-detection (human sB7-DC Biotin-dAb). PN: B113383B. One vial containing 100 µL of biotinylated anti-human sB7-DC.

Lyophilized Standard Mix-Human Group B, 9-Plex. PN: HG9009B. One vial containing lyophilized recombinant human sCEACAM-1, sALCAM, sSLAMF4, sHVEM, sB7-DC, sB7-H1, sEpCAM, sBLAME and sEPOR.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human Human PD-L2/B7-DC/sCD273. Can be multiplexed with other analytes in Human Group 9.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 20 pg/mL

  • Quantitation range:

  • LLOQ: < 40 pg/mL

  • ULOQ: > 10,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 9: Negligible

  • Sample volume: 15 µL/test

Description:

Programmed Death Ligand 2 (PD-L2), also known as B7-DC and CD273. CD273 is a single-pass type 1 membrane protein belonging to the immunoglobulin receptor superfamily, and is a ligand for CD279 (PD-1). CD273 is highly expressed in the tissues of the heart, liver, lung, placenta and pancreas. It is expressed in dendritic cells, and its expression can be induced on inflammatory macrophages when activated by interleukin 4 or Th2 cells. CD273 functions to inhibit T-cell proliferation by blocking cell cycle progression and cytokine production, and is particularly important in the maintenance of self-tolerance. Diseases associated with PDCD1LG2 include cysticercosis.

References:

  1. Zhang Y, et al. (2006) Regulation of T cell activation and tolerance by PDL2. PNAS 103:11695-700 2.

  2. LaGier, A.J. & Pober, J.S. (2006) Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 67:568-78 3.

  3. Ishida Y, et al. (1992) Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 11:3887-95. .

  4. Holling TM, et al. (2004) Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 65:282-90