• PN: B117376

COMPONENTS

45x Ab-conjugated beads (S5P3 - Human Pro-BDNF Ab-bead). PN: B117376A. One vial containing 100 µL of anti-human Pro-BDNF conjugated to AimPlex Bead S5P8.

25x Biotin-detection Ab (Human Pro-BDNF Biotin-dAb). PN: B117376B. One vial containing 100 µL of biotinylated anti- Human Pro-BDNF.

Lyophilized Standard Mix - Human Pro-BDNF. PN: B117376S. One vial containing lyophilized Pro-BDNF.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Pro-BDFN. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

NOTE: This assay cannot be multiplexed with the AimPlex BDNF assay.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 50 pg/mL

Quantitation range:

LLOQ: <100 pg/mL

ULOQ: > 20,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Sample volume: 15 µL/test

Cross-reactivity:  < 2% cross-reactivity with human BDNF at 5,000pg/mL.

Description:

Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors, which are related to the canonical Nerve Growth Factor. Neurotrophic factors are found in the brain and the periphery. BDNF has been recognized as a key regulator of synapse development and plasticity. This is because BDNF is the only neurotrophin indisputably secreted in an activity-dependent manner. Indeed, activity-dependent secretion of BDNF has been shown to be critical for hippocampus-dependent memory in human. Like all neurotrophins, BDNF is initially synthesized as a precursor (proBDNF), which is subsequently cleaved to generate mature (m)BDNF. proBDNF interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR), whereas mBDNF selectively binds and activates the receptor tyrosine kinase TrkB. Cumulative evidence supports a “yin-yang hypothesis,” in which pro- and mBDNF elicit opposite biological effects by activating two distinct receptor systems. For example, proBDNF, if not processed, promotes long-term depression (LTD) through the activation of p75NTR in the hippocampus.

References:

1.      Binder DK, Scharfman HE (September 2004). "Brain-derived neurotrophic factor". Growth Factors. 22 (3): 123–31. doi:10.1080/08977190410001723308. PMC 2504526 Freely accessible. PMID 15518235.

2.      Poo MM. Neurotrophins as synaptic modulators. Nat Rev Neurosci. 2001;2:24–32. [PubMed]

3.      Lu B. BDNF and activity-dependent synaptic modulation. Learn Mem. 2003;10:86–98. [PMC free article] [PubMed]

4.      Egan MF, et al. The BDNF val66met polymorphism affects activity-dependent secretion of BDNF and human memory and hippocampal function. Cell. 2003;112:257–269. [PubMed]

5.      Lu B. Pro-region of neurotrophins: Role in synaptic modulation. Neuron. 2003;39:735–738. [PubMed]

6.      Heinonen I, Kalliokoski KK, Hannukainen JC, Duncker DJ, Nuutila P, Knuuti J (November 2014). "Organ-specific physiological responses to acute physical exercise and long-term training in humans". Physiology. 29 (6): 421–36. doi:10.1152/physiol.00067.2013. PMID 25362636.