PN: B117694

COMPONENTS

45x Ab-conjugated beads

S5P3- Human Reg3A Ab-bead). PN: B117694A. One vial containing 100 µL of anti-human Reg3A conjugated to AimPlex Bead S5P3.

25x Biotin-detection Ab (Human Reg3A Biotin-dAb). PN: B117694B. One vial containing  100 µL of biotinylated anti-Human Reg3A.

Lyophilized Standard -Human Reg3A. PN: B117694S. One vial containing lyophilized Reg3A. 

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Reg3A.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 15 pg/mL

Quantitation range:

LLOQ: < 30 pg/mL

ULOQ: > 10,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Sample volume: 15 µL/test

Description:

Regenerating islet-derived protein 3 alpha (Reg3A), formerly known as HIP/PAP (Hepatocarcinoma-Intestine-Pancreas/Pancreatitis-Associated Protein) and peptide 23, is a pancreatic secretory protein that may be involved in cell proliferation or differentiation. Increases of Reg3A were observed during pancreatic inflammation and liver carcinogenesis. Reg3A is also a bactericidal C-type lectin that is constitutively produced in the intestine and has antibacterial properties against Gram-positive bacteria mediated by binding to surface-exposed carbohydrate moieties of bacterial peptidoglycan.

Diseases associated with REG3A include Pancreatitis and Acute Pancreatitis.

References:

1.      Chakraborty C, Sharma S, Katsumata N, Murphy LJ, Schroedter IC, Robertson MC, Shiu RP, Friesen HG (Jun 1995). Plasma clearance, tissue uptake and expression of pituitary peptide 23/pancreatitis-associated protein in the rat. Journal of Endocrinology. PMID 7636430.

2.      Dusetti NJ, Frigerio JM, Fox MF, Swallow DM, Dagorn JC, Iovanna JL (Jun 1994). Molecular cloning, genomic organization, and chromosomal localization of the human pancreatitis-associated protein (PAP) gene. Genomics. 19 (1): 108–14. doi:10.1006/geno.1994.1019. PMID 8188210.

3.      Cash, Heather; et.al.. (2006-08-25). Symbiotic bacteria direct expression of an intestinal bactericidal lectin. Science. 313 (5790): 1126–1130. doi:10.1126/science.1127119. ISSN 1095-9203. PMC 2716667 Freely accessible. PMID 16931762.

4.      Vaishnava, Shipra; et.al. (2011-10-14). The antibacterial lectin RegIIIγ promotes the spatial segregation of microbiota and host in the intestine. Science. 334 (6053): 255–258. doi:10.1126/science.1209791. ISSN 0036-8075. PMC 3321924 Freely accessible. PMID 21998396