• PN: B112521

COMPONENTS

45x Ab-conjugated beads (S4P12- Human TARC Ab-bead). PN: B112521A. One vial containing 100 µL of anti-Human TARC conjugated to AimPlex Bead S4P12.

25x Biotin-detection Ab (Human TARC Biotin-dAb). PN: B112521B. One vial containing 100 µL of biotinylated anti-Human TARC.

Lyophilized Standard Mix-Human Group 7, Panel B, 7-Plex. PN: HG72006. One vial containing lyophilized recombinant NAP-2, PARC, sPD-1, PF4, SDF-1, TARC, and TECK.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human CCL17/SCYA17/TARC. Can be multiplexed with other analytes in Human Group 7.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 5 pg/mL

Quantitation range:

LLOQ: < 10 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 7: Negligible

Sample volume: 15 µL/test

Description:

Chemokine (C-C motif) ligand 17 (CCL17) is a small cytokine belonging to the CC chemokine family is also known as small inducible cytokine A17 (SYCA17) andthymus and activation regulated chemokine (TARC). CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells. This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4. The gene for CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1. Diseases associated with CCL17 include mycosis fungoides and paragonimiasis.

References:

1.      Imai T, Yoshida T, Baba M, Nishimura M, Kakizaki M, Yoshie O (1996). "Molecular cloning of a novel T cell-directed CC chemokine expressed in thymus by signal sequence trap using Epstein-Barr virus vector". J. Biol. Chem. 271 (35): 21514–21. doi:10.1074/jbc.271.35.21514. PMID 8702936.

2.      Imai T, Baba M, Nishimura M, Kakizaki M, Takagi S, Yoshie O (1997). "The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4". J. Biol. Chem. 272 (23): 15036–42. doi:10.1074/jbc.272.23.15036. PMID 9169480.

3.      Nomiyama H, Imai T, Kusuda J, Miura R, Callen DF, Yoshie O (1997). "Assignment of the human CC chemokine gene TARC (SCYA17) to chromosome 16q13". Genomics 40 (1): 211–3. doi:10.1006/geno.1996.4552. PMID 9070951.

4.      Nomiyama H, Imai T, Kusuda J, Miura R, Callen DF, Yoshie O (1998). "Human chemokines fractalkine (SCYD1), MDC (SCYA22) and TARC (SCYA17) are clustered on chromosome 16q13". Cytogenet. Cell Genet. 81 (1): 10–1. doi:10.1159/000015000. PMID 9691168.