• PN: B111452

COMPONENTS

45x Ab-conjugated beads (S4P11 - human TIM-1 Ab-bead). PN: B111452A. One vial containing 100 µL of anti-human TIM-1 conjugated to AimPlex Bead S4P11.

25x Biotin-detection Ab (human TIM-1 Biotin-dAb). PN: B111452B. One vial containing 100 µL of biotinylated anti-human TIM-1.

Lyophilized Standard Mix-Human Group 3 Panel A, 8-Plex. PN: HG30008. One vial containing lyophilized recombinant human Fractalkine, LIF, TNFβ, VEGF-D, I-TAC, MIP-1α, MIP-1β, and TIM-1.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human TIM-1/KIM-1/HAVCR.  Can be multiplexed with other analytes in Human Group 3.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 5 pg/mL

Quantitation range:

LLOQ: < 10 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 3: Negligible

Sample volume: 15 µL/test

Description:

T-cell immunoglobulin and mucin domain 1 (TIM-1), also known as Hepatitis A virus cellular receptor 1 (HAVcr-1), is a protein that in humans is encoded by the HAVCR1 gene. The hepatitis A virus cellular receptor 1 is a member of the TIM (T cell transmembrane, immunoglobulin, and mucin) gene family, which plays critical roles in regulating immune cell activity especially regarding the host response to viral infection. TIM-1 is also involved in allergic response, asthma, and transplant tolerance. Members of the TIM gene family including TIM-1 participate in host immune response. The mouse TIM gene family contains eight members (TIM-1-8) while only three TIM genes (TIM-1, TIM-3, and TIM-4) have been identified in humans. TIM-1 is preferentially expressed on Th2 cells and has been identified as a stimulatory molecule for T-cell activation. TIM-1 has been identified as the cellular receptor of hepatitis A virus (HAV). The mucin domain and IgV domain were critical for virus uncoating and infectivity. By using an expression cloning library, IgA has been demonstrated as a specific ligand of TIM-1. The association of TIM-1 and IgA was able to enhance the virus-receptor interaction. Diseases associated with HAVCR1 include atopy and hepatitis.

References:

1.      Feigelstock D, Thompson P, Mattoo P, Zhang Y, Kaplan GG (Aug 1998). "The human homolog of HAVcr-1 codes for a hepatitis A virus cellular receptor". J Virol 72 (8): 6621–8. PMC 109848. PMID 9658108.

2.      McIntire JJ, Umetsu SE, Akbari O, Potter M, Kuchroo VK, Barsh GS, Freeman GJ, Umetsu DT, DeKruyff RH (Nov 2001). "Identification of Tapr (an airway hyperreactivity regulatory locus) and the linked Tim gene family". Nat Immunol 2 (12): 1109–16. doi:10.1038/ni739. PMID 11725301.

3.      Umetsu SE, Lee WL, McIntire JJ, Downey L, Sanjanwala B, Akbari O, Berry GJ, Nagumo H, Freeman GJ, Umetsu DT, DeKruyff RH (May 2005). "TIM-1 induces T cell activation and inhibits the development of peripheral tolerance". Nat. Immunol. 6 (5): 447–54. doi:10.1038/ni1186. PMID 15793575.

4.      Tami C, Silberstein E, Manangeeswaran M, Freeman GJ, Umetsu SE, DeKruyff RH, Umetsu DT, Kaplan GG (April 2007). "Immunoglobulin A (IgA) is a natural ligand of hepatitis A virus cellular receptor 1 (HAVCR1), and the association of IgA with HAVCR1 enhances virus-receptor interactions". J. Virol. 81 (7): 3437–46. doi:10.1128/JVI.01585-06. PMC 1866050. PMID 17229699.