• PN: B115323

COMPONENTS

45x Ab-conjugated beads (S4P6 - Human TIMP-1 Ab-bead). PN: B115323A. One vial containing 100 µL of anti-Human TIMP-1 conjugated to AimPlex Bead S4P6.

25x Biotin-detection (Human TIMP-1 Biotin-dAb). PN: B115323B. One vial containing 100 µL of biotinylated anti-Human TIMP-1.

Lyophilized Standard Mix-Human Group 8, Panel A, 7-Plex. PN: HG81007. One vial containing lyophilized recombinant BMP-2, BMP-4, IGFBP-3, IGFBP-5, IGFBP-6, TIMP-1, and TIMP-2.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Human TIMP-1. Can be multiplexed with other analytes in Human Group 8.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 10 pg/mL

Quantitation range:

LLOQ: < 20 pg/mL

ULOQ: > 10,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 8: Negligible

Sample volume: 15 µL/test

Description:

TIMP metallopeptidase inhibitor 1 (TIMP-1), also known as TIMP1, is a glycoprotein that is expressed from the several tissues of organisms. TIMP-1 protein is a member of the TIMP family. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Diseases associated with TIMP1 include oral submucous fibrosis and Sorsby Fundus Dystrophy.

References:

1.      Reichenstein M, Reich R, LeHoux JG, Hanukoglu I (February 2004). "ACTH induces TIMP-1 expression and inhibits collagenase in adrenal cortex cells". Mol. Cell. Endocrinol. 215 (1-2): 109–14. doi:10.1016/j.mce.2003.11.011. PMID 15026182.

2.      Ma J (Dec 2013). "Upregulated TIMP-1 correlates with poor prognosis of laryngeal squamous cell carcinoma.". Int J Clin Exp Pathol. 15 (1): 246–54. PMC 3885479. PMID 24427345.

3.      Tarhini AA (Jan 2014). "A four-marker signature of TNF-RII, TGF-α, TIMP-1 and CRP is prognostic of worse survival in high-risk surgically resected melanoma..". J Transl Med. 12. doi:10.1186/1479-5876-12-19. PMC 3909384. PMID 24457057.