96 Tests PN: A111212
45x Ab-conjugated beads (S4P4 - human TNFβ Ab-bead). PN: A111212A. One vial containing 100 µL of anti-human TNFβ conjugated to AimPlex Bead S4P4.
25x Biotin-detection Ab (human TNFβ Biotin-dAb). PN: A111212B. One vial containing 100 µL of biotinylated anti-human TNFβ.
Lyophilized Standard Mix-Human Group 3 Panel A, 8-Plex. PN: HG30008. One vial containing lyophilized recombinant human Fractalkine, LIF, TNFβ, VEGF-D, I-TAC, MIP-1α, MIP-1β, and TIM-1. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
Application: Optimal antibody pair and antigen standard for assaying human TNFβ/TNFSF1/LTA. Can be multiplexed with other analytes in Human Group 3. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
Storage: 2-8 C in the dark.
Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 5 pg/mL
LLOQ: < 10 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Human Group 3: Negligible
Sample volume: 15 µL/test
Tumor necrosis factor-beta (TNF-β), also known as Tumor Necrosis Factor Receptor Superfamily Member 1A (TNFSF1A) and Lymphotoxin-alpha (LTA), is a cytokine produced by lymphocytes and a member of the tumor necrosis factor family. LTA is highly inducible, secreted, and exists as homotrimeric molecule. LTA forms heterotrimers with lymphotoxin-beta, which anchors lymphotoxin-alpha to the cell surface. LTA mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. LTA is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. In TNF-β knockout mice, all Peyer's patches and lymph nodes will fail to develop indicating TNF-β's importance in immunological development. Diseases associated with TNFRSF1A include multiple sclerosis 5 and periodic fever
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