- PN: A113308
45x Ab-conjugated beads (S5P8 - Human TNFRII Ab-bead). PN: A113308A. One vial containing 100 µL of anti-Human TNFR2 conjugated to AimPlex Bead S5P8.
25x Biotin-detection Ab (Human TNFRII Biotin-dAb). PN: A113308B. One vial containing 100 µL of biotinylated anti-Human TNFR2.
Lyophilized Standard Mix-Human Group 6, Panel B, 12-Plex. PN: HG62013. One vial containing lyophilized recombinant Eotaxin-2, IGFBP-1, IGFBP-7, MCP-4, MDC, MIP-1δ, MIP-3α, MIP-3β, IL-6Rα, IL-1RII, Gp130, and TNFRII. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
STORAGE: 2-8 C in the dark.
IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
APPLICATION: Optimal antibody pair and antigen standard for assaying human TNFRSF1B/sCD120b/sTNF-R2. Can be multiplexed with other analytes in Human Group 6. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 10 pg/mL
LLOQ: < 20 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Human Group 6: Negligible
Sample volume: 15 µL/test
Tumor necrosis factor receptor 2 (TNF-R2), also known as tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) and CD120b, is a membrane receptor that binds tumor necrosis factor-alpha (TNFα). TNFR2 is a member of the tumor necrosis factor receptor superfamily, which also contains TNFRSF1A. TNFR2 and TNF-receptor 1 form a hetero-complex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating anti-oxidative pathways. Diseases associated with TNFRSF1B include palindromic rheumatism and post-transplant lymphoproliferative disease.
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