PN: C190181
Components:
1x Ab-conjugated beads (premixed). PN: C190181A. One bottle containing 4.7 mL of the following AimPlex antibody-bead conjugates:
S4P2 - anti-H IL-2RA
S4P3 - anti-H IL-1beta
S4P4 - anti-H IL-6
S4P6 - anti-H IL-8
S4P7 - anti-H IL-10
S4P9 - anti-H MIP-1alpha
S4P11 - anti-H MCP-1
S4P12 - anti-H IL-12p70
S5P2 - anti-H IFNgamma
S5P3 - anti-H GM-CSF
S5P4 - anti-H IL-4
S5P5 - anti-H IL-15
S5P6 - anti-H Reg3A
S5P7 - anti-H Elafin
S5P8 - anti-H ST-2
S5P9 - anti-H IL-17A
S5P10 - anti-H TNFRI
S5P12 - anti-H Granzyme B
2x Biotin-detection Ab (premixed). PN: C190181B. One vial containing 1.25 mL of the following biotinylated antibodies.
Biotin - anti-H IL-2RA
Biotin - anti-H IL-1beta
Biotin - anti-H IL-6
Biotin - anti-H IL-8
Biotin - anti-H IL-10
Biotin - anti-H MIP-1alpha
Biotin - anti-H MCP-1
Biotin - anti-H IL-12p70
Biotin - anti-H IFNgamma
Biotin - anti-H GM-CSF
Biotin - anti-H IL-4
Biotin - anti-H IL-15
Biotin - anti-H Reg3A
Biotin - anti-H Elafin
Biotin - anti-H ST-2
Biotin - anti-H IL-17A
Biotin - anti-H TNFRI
Biotin - anti-H Granzyme B
2x NR dAb Diluent. PN: 07NDX21. One vial containing 1.25 mL of biotin detection antibody diluent.
Lyophilized Standard Mix- Human CAR-T CRS 18-Plex Panel. PN: HG19318. One vial containing lyophilized recombinant Elafin, GM-CSF, Granzyme B, IFNγ, IL-1β, IL-2RA, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-15, IL-17A, MCP-1, MIP-1α, Reg3A, ST-2, TNFRI.
Standard Info Sheet: Indicates reconstitute volume and standard concentrations.
Basic Kit for Premixed Panels:
1x Streptavidin-PE (SAPE). PN: 07SP0011. One bottle containing 2.5 mL of 1x SAPE.
10x Reading Buffer. PN: 07RB1. One bottle containing 4 mL of 10x buffer.
10x Wash Buffer. PN: 07WB1. One vial containing 15 mL of 10x buffer.
PCR 8-tube Strip. PN: 07P8T1. 2 strips.
Filter Plate and Lid. PN: 07FPL1. 1 96- well filter plate and 1 plate lid.
Plate Seals. PN: 07PSR1. 4 sealers.
Application: Optimal antibody pairs and antigen standards for assaying the CAR-T CRS 18-Plex Panel. To be used in conjunction with the AimPlex Basic Kit for Premixed Panels - 96 Tests (PN: U100005) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
Storage: 2-8 C in the dark.
Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
Assay Specifications:
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LODs): Elafin, < 20 pg/mL; GM-CSF, < 5 pg/mL; Granzyme B, < 10 pg/mL; IFNγ, < 3 pg/mL; IL-1β, < 5 pg/mL; IL-2RA, < 150 U/mL; IL-4, < 1 pg/mL; IL-6, < 5 pg/mL; IL-8, < 1 pg/mL; IL-10, < 2 pg/mL; IL-12p70, < 3 pg/mL; IL-15, < 5 pg/mL; IL-17A, < 3 pg/mL; MCP-1, < 2 pg/mL; MIP-1α, < 2 pg/mL; Reg3A, < 15 pg/mL; ST-2, < 2 pg/mL; TNFRI, < 5 pg/mL.
LLOQs: Elafin, < 50 pg/mL; GM-CSF, < 10 pg/mL; Granzyme B, < 20 pg/mL; IFNγ, < 10 pg/mL; IL-1β, < 10 pg/mL; IL-2RA, < 300 U/mL; IL-4, < 3 pg/mL; IL-6, < 10 pg/mL; IL-8, < 1 pg/mL; IL-10, < 5 pg/mL; IL-12p70, < 10 pg/mL; IL-15, < 10 pg/mL; IL-17A, < 10 pg/mL; MCP-1, < 5 pg/mL; MIP-1α, < 5 pg/mL; Reg3A, < 30 pg/mL; ST-2, < 5 pg/mL; TNFRI, < 10 pg/mL.
ULOQs: Elafin, > 10,000 pg/mL; GM-CSF, > 5,000 pg/mL; Granzyme B, > 5,000 pg/mL; IFNγ, > 2,000 pg/mL; IL-1β, > 5,000 pg/mL; IL-2RA, > 150,000 U/mL; IL-4, > 1,000 pg/mL; IL-6, > 5,000 pg/mL; IL-8, > 1,000 pg/mL; IL-10, > 5,000 pg/mL; IL-12p70, > 2,000 pg/mL; IL-15, > 5,000 pg/mL; IL-17A, > 1,000 pg/mL; MCP-1, > 5,000 pg/mL; MIP-1α, > 2,000 pg/mL; Reg3A, > 10,000 pg/mL; ST-2, > 5,000 pg/mL; TNFRI, > 5,000 pg/mL.
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in the Panel: Negligible
Sample volume: 15 µL/test
For Research Use Only. Not for use in diagnostic procedures.