96 Tests PN: B111424

Components:

45x Ab-conjugated beads (S5P7 - human IL-37/IL-1F7 -bead). PN: B111424A. One vial containing 100 µL of anti-human IL-37/IL-1F7 conjugated to AimPlex Bead S5P7.

25x Biotin-detection Ab (human IL-37/IL-1F7 Biotin-dAb). PN: B111424B. One vial containing 100 µL of biotinylated anti-human IL-37/IL-1F7.

Lyophilized Standard - Human IL-37/IL-1F7. PN: B111424S. One vial containing lyophilized IL-37/IL-1F7.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

Application: Optimal antibody pair and antigen standard for assaying human IL-37/IL-1F7. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 10 pg/mL

  • Quantitation range:

  • LLOQ: < 20 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

Interleukin 37 (IL-37) (Accession AAG14421), also known as Interleukin-1 family member 7 (IL-1F7), is an anti-inflammatory cytokine important for the downregulation of pro-inflammatory cytokine production, the suppression of tumor cell growth, and antimicrobial responses.  Breast cancer and ovarian cancer are associated with elevated expression of IL-37. Colon cancer, lung cancer, Multiple Myeloma, and Hepatoma Carcinoma were correlated with decreased expression of IL-37 expression in affected areas.

References:

1.    Wang L, et al. Interleukin-37: A crucial cytokine with multiple roles in disease and potentially clinical therapy. Oncology Letters. 15 (4): 4711–4719. doi:10.3892/ol.2018.7982. PMC 5840652. PMID 29552110.

2.    Mei Y, et al. IL-37: An anti-inflammatory cytokine with antitumor functions". Cancer Reports. 2 (2): e1151. doi:10.1002/cnr2.1151. PMC 7941439. PMID 32935478.