• PN: B175604

COMPONENTS

45x Ab-conjugated beads (S5P2 - Human Keratin Ab-bead). PN: B175604A. One vial containing 100 µL of anti-human Klotho conjugated to AimPlex Bead S4P6.

25x Biotin-detection Ab (Human Keratin Biotin-dAb). PN: B175604B. One vial containing 100 µL of biotinylated anti- Human Klotho.

Lyophilized Standard Mix - Human Klotho. PN: B175604S. One vial containing lyophilized Klotho.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Human Klotho2/KLA/KL. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 10 pg/mL

Quantitation range:

LLOQ: <20 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Sample volume: 15 µL/test

Description:

Keratin, type I cytoskeletal 19 also known as cytokeratin-19 (CK-19) or keratin-19 (KRT19) is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Due to its high sensitivity, KRT19 is the most used marker for the detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients. Keratin 19 is often used together with keratin 8 and keratin 18 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.

References:

1.       Schweizer J, Bowden PE, Coulombe PA, Langbein L, Lane EB, Magin TM, Maltais L, Omary MB, Parry DA, Rogers MA, Wright MW (Jul 2006). "New consensus nomenclature for mammalian keratins". The Journal of Cell Biology 174 (2): 169–74. doi:10.1083/jcb.200603161. PMC 2064177. PMID 16831889.

2.       "Serum CYFRA 21-1 (cytokeratin-19 fragments) is a useful tumour marker for detecting disease relapse and assessing treatment efficacy in breast cancer". Retrieved August 6, 2014.

3.       Lacroix M (Dec 2006). "Significance, detection and markers of disseminated breast cancer cells". Endocrine-Related Cancer 13 (4): 1033–67. doi:10.1677/ERC-06-0001. PMID 17158753.