96 Tests PN: B117712
Components:
45x Ab-conjugated beads (S5P9 - human S100B Ab-bead). PN: B117712A. One vial containing 100 µL of S100B conjugated to AimPlex Bead S5P9.
25x Biotin-detection Ab (human S100B Biotin-dAb). PN: B117712B. One vial containing 100 µL of biotinylated anti-human S100B.
Lyophilized Standard -Human S100B. PN: B117712S. One vial containing lyophilized S1100B.
Application: Optimal antibody pair and antigen standard for assaying human S100B. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
Storage: 2-8 C in the dark.
Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
For Research Use Only. Not for use in diagnostic procedures.
Assay Specifications:
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 10 pg/mL
Quantitation range:
LLOQ: < 20 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Sample volume: 15 µL/test
Description:
S100B/S100 calcium-binding protein B (Accession P04271) is a protein primarily expressed in the brain by astrocytes. It interacts with the Receptor for Advanced Glycation End Products (RAGE) and plays a regulatory role in neuroprotective pathways. It is associated with the progression of neuroinflammatory conditions, including Alzheimer's disease, schizophrenia, and epilepsy.
References:
Yu WH et. al. S100beta interaction with tau is promoted by zinc and inhibited by hyperphosphorylation in Alzheimer's disease. J. Neurosci. 2001, 21: 2240–6.
Sen J and Belli A. S100B in neuropathologic states: the CRP of the brain?. J. Neurosci. Res. 2007, 85: 1373–80.