• PN: B116657

COMPONENTS

45x Ab-conjugated beads (S5P6- Human Uteroglobin Ab-bead). PN: B116657A. One vial containing 100 µL of anti-human Uteroglobin conjugated to AimPlex Bead S5P6.

25x Biotin-detection Ab (Human Uteroglobin Biotin-dAb). PN: B116657B. One vial containing 100 µL of biotinylated anti- Human Uteroglobin.

Lyophilized Standard Mix - Human Uteroglobin. PN: B116657S. One vial containing lyophilized Uteroglobin.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Uteroglobin. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 50 pg/mL

Quantitation range:

LLOQ: < 100 pg/mL

ULOQ: > 50,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Sample volume: 15 µL/test

Description:

Uteroglobin, is also known as secretoglobin family 1A member 1 (SCGB1A1), club-cell-specific 10 kD protein (CC10), and Clara cell specific protein (CC16). It is a member of the secretoglobin family of small, secreted, disulfide-bridged dimeric proteins.  Uteroglobin functions as an immunosuppressant and plays an important protective role as an anti-inflammatory agent in the lung.  Studies found that Its levels were significantly lower in serum samples of smokers and chronic obstructive pulmonary disease (COPD) patients.

References:

1.         Laukaitis CM, Karn RC (2005). "Evolution of the secretoglobins: a genomic and proteomic view". Biological Journal of the Linnean Society. 84 (3): 493–501. doi:10.1111/j.1095-8312.2005.00450.x

 2.         Arsalane K, Broeckaert F, Knoops B, Wiedig M, Toubeau G, Bernard A (2000). “Clara cell specific protein (CC16) expression after acute lung inflammation induced by intratracheal lipopolysaccharide administration”. Am J Respir Crit Care Med. 161:1624–1630. doi: 10.1164/ajrccm.161.5.9812157.

3.         Shijubo N, Itoh Y, Yamaguchi T, Shibuya Y, Morita Y, Hirasawa M, et al. (1997) Serum and BAL Clara cell 10 kDa protein (CC10) levels and CC10-positive bronchiolar cells are decreased in smokers. Eur Respir J. 10(5):1108.

4.         Lomas DA, Silverman EK, Edwards LD, Miller BE, Coxson HO, Tal-Singer R. (2008) Evaluation of serum CC-16 as a biomarker for COPD in the ECLIPSE cohort. Thorax. 63(12):1058.