1x Ab-conjugated beads (premixed). PN: C191032A. One vial containing 5 mL of the following AimPlex antibody-bead conjugates:
S4P3 - anti-human TNFα
S5P6 - anti-human IL-6
S5P10 - anti-human IL-10
2x Biotin-detection Ab (premixed). PN: C191032B. One vial containing 1.5 mL of the following biotinylated antibodies.
Biotin - anti-human TNFα
Biotin - anti-human IL-6
Biotin - anti-human IL-10
2x NR dAb Diluent. PN: 07NDX21. One vial containing 1.5 mL of biotin detection antibody diluent
Lyophilized Standard Mix- Human T Helper Cytokine 3-Plex Panel 1. PN: HG10032. One vial containing lyophilized recombinant IL-6, IL-10, and TNFα.
Standard Info Sheet: Indicates reconstitute volume and standard concentrations.
STORAGE: 2-8oC in the dark.
IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
APPLICATION: Optimal antibody pairs and antigen standards for assaying T Helper Cytokine 3-Plex Panel 2. To be used in conjunction with the AimPlex Basic Kit for Premixed Panels - 96 Tests(PN: U100005) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LODs): IL-6, < 5 pg/mL; IL-10, < 2 pg/mL; TNFα, < 1 pg/mL.
LLOQs: IL-6, < 10 pg/mL; IL-10, < 5 pg/mL; TNFα, < 2 pg/mL.
ULOQs: IL-6, > 5,000 pg/mL; IL-10, > 5,000 pg/mL; TNFα, > 1,000 pg/mL.
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in the Panel: Negligible
Sample volume: 15 µL/test
For Research Use Only. Not for use in diagnostic procedures.