96 Tests PN: B211221

Components:

45x Ab-conjugated beads (S5P4 - Mouse BTC Ab-bead). PN: B211221A. One vial containing 100 µL of anti-mouse BTC conjugated to AimPlex Bead S5P4.

25x Biotin-detection Ab (Mouse BTC Biotin-dAb). PN: B211221B. One vial containing 100 µL of biotinylated anti-mouse BTC.

Lyophilized Standard Mix - Mouse Group 3 Panel A, 8-Plex. PN: MG3008A. One vial containing lyophilized recombinant mouse BTC, IL-1RA, IL-9, IL-23p19, IL-25, Leptin, LIF and VEGF.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying human Mouse BTC/Betacellulin. Can be multiplexed with other analytes in Mouse Group 3. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only. Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 2 pgmL

  • Quantitation range:

  • LLOQ: < 5 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Human Group 1: Negligible

  • Sample volume: 15 µL/test

Description:

Betacellulin, or BTC, is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. BTC was originally identified as a growth-promoting factor in mouse pancreatic β-cell carcinoma cell line and has since been identified in humans. BTC is synthesized in a wide range of adult tissues and in many cultured cells, including smooth muscle cells and epithelial cells.

References:

  1. Kim HS, Shin HS, Kwak HJ, Cho CH, Lee CO, Koh GY (February 2003). "Betacellulin induces angiogenesis through activation of mitogen-activated protein kinase and phosphatidylinositol 3'-kinase in endothelial cell". FASEB J. 17 (2): 318–20. doi:10.1096/fj.02-0570fje. PMID 12475887.

  2. Yamamoto T, Akisue T, Marui T; et al. (2004). "Expression of betacellulin, heparin-binding epidermal growth factor and epiregulin in human malignant fibrous histiocytoma.". Anticancer Res. 24 (3b): 2007–10. PMID 15274392.

  3. Nakagawa T, Furuta H, Sanke T; et al. (2005). "Molecular scanning of the betacellulin gene for mutations in type 2 diabetic patients.". Diabetes Res. Clin. Pract. 68 (3): 188–92. doi:10.1016/j.diabres.2004.09.019. PMID 15936459.