• PN: B212290

COMPONENTS

45x Ab-conjugated beads (S4P4 - Mouse MEC Ab-bead). PN: B212290A. One vial containing 100 µL of anti-mouse MEC conjugated to AimPlex Bead S4P4.

25x Biotin-detection Ab (Mouse MEC Biotin-dAb). PN: B212290B. One vial containing 100 µL of biotinylated anti-mouse MEC.

Lyophilized Standard Mix - Mouse MEC. PN: MMECAG. One vial containing lyophilized recombinant mouse MEC.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Mouse CCL28/MEC/CCK-1. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 20 pg/mL

Quantitation range:

LLOQ: < 40 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Sample volume: 15 µL/test

Description:

Chemokine (C-C motif) ligand 28 (CCL28), also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 is expressed by columnar epithelial cells in the gut, lung, breast and the salivary glands and drives the mucosal homing of T and B lymphocytes that express CCR10, and the migration of eosinophils expressing CCR3. This chemokine is constitutively expressed in the colon, but its levels can be increased by pro-inflammatory cytokines and certain bacterial products implying a role in effector cell recruitment to sites of epithelial injury. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans.

References:

1.      Rodriguez MW, Paquet AC, Yang YH, Erle DJ (July 2004). "Differential gene expression by integrin beta 7+ and beta 7- memory T helper cells". BMC Immunology. 5: 13. doi:10.1186/1471-2172-5-13. PMC 476736. PMID 15236665. 

2.      Kunkel EJ, Kim CH, Lazarus NH, Vierra MA, Soler D, Bowman EP, Butcher EC (April 2003). "CCR10 expression is a common feature of circulating and mucosal epithelial tissue IgA Ab-secreting cells". The Journal of Clinical Investigation. 111 (7): 1001–10. doi:10.1172/JCI17244. PMC 152588. PMID 12671049. 

3.      John AE, Thomas MS, Berlin AA, Lukacs NW (February 2005). "Temporal production of CCL28 corresponds to eosinophil accumulation and airway hyperreactivity in allergic airway inflammation". The American Journal of Pathology. 166 (2): 345–53. doi:10.1016/S0002-9440(10)62258-4. PMC 1602329. PMID 15681819. 

4.      Hieshima K, Ohtani H, Shibano M, Izawa D, Nakayama T, Kawasaki Y, Shiba F, Shiota M, Katou F, Saito T, Yoshie O (February 2003). "CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity". Journal of Immunology. 170 (3): 1452–61. doi:10.4049/jimmunol.170.3.1452. PMID 12538707.