• PN: B213408

COMPONENTS

45x Ab-conjugated beads (S5P4 - Mouse sICAM-1 Ab-bead). PN: B213408A. One vial containing 100 µL of anti-mouse sICAM-1 conjugated to AimPlex Bead S5P4.

25x Biotin-detection Ab (Mouse sICAM-1 Biotin-dAb). PN: B213408B. One vial containing 100 µL of biotinylated anti-mouse sICAM-1.

Lyophilized Standard Mix - Mouse Group 5 Panel A, 9-Plex. PN: MG5009A. One vial containing lyophilized recombinant sCD10, sCD14, sICAM, sE-selectin, sL-selectin, sP-selectin, sVCAM, sCD30L, and sCD40L.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying mouse ICAM-1/sCD54. Can be multiplexed with other analytes in Mouse Group 5.  To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 100 pg/mL

Quantitation range:

LLOQ: < 200 pg/mL

ULOQ: > 20,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 5: Negligible

Sample volume: 15 µL/test

Description:

ICAM-1 (Intercellular Adhesion Molecule 1) also known as CD54 is a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by rhinovirus as a receptor. It is a type of intercellular adhesion molecule continuously present in low concentrations in the membranes of leukocytes and endothelial cells. Upon cytokine stimulation, the concentrations greatly increase. ICAM-1 can be induced by interleukin-1 (IL-1) and tumor necrosis factor (TNF) and is expressed by the vascular endothelium, macrophages, and lymphocytes. ICAM-1 is a ligand for LFA-1 (integrin), a receptor found on leukocytes. When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 and then transmigrate into tissues. LFA-1 has also been found in a soluble form, which seems to bind and block ICAM-1. ICAM-1 has been implicated in subarachnoid hemorrhage (SAH). Levels of ICAM-1 are shown to be significantly elevated in patients with SAH over control subjects in many studies.[13][14] While ICAM-1 has not been shown to be directly correlated with cerebral vasospasm, a secondary symptom that affects 70% of SAH patients, treatment with anti-ICAM-1 reduced the severity of vasospasm.  ICAM-1 expressed by respiratory epithelial cells is also the binding site for rhinovirus, the causative agent of most common colds. ICAM-1 has an important role in ocular allergies recruiting pro-inflammatory lymphocytes and mast cells promoting a type I hypersensitivity reaction.

References:

1.       Carlson M, Nakamura Y, Payson R, O'Connell P, Leppert M, Lathrop GM, Lalouel JM, White R (May 1988). "Isolation and mapping of a polymorphic DNA sequence (pMCT108.2) on chromosome 18 [D18S24]". Nucleic Acids Research 16 (9): 4188. doi:10.1093/nar/16.9.4188. PMC 336612. PMID 2453850.

2.       Katz FE, Parkar M, Stanley K, Murray LJ, Clark EA, Greaves MF (Jan 1985). "Chromosome mapping of cell membrane antigens expressed on activated B cells". European Journal of Immunology 15 (1): 103–6. doi:10.1002/eji.1830150121. PMID 3871395.

3.       Bella J, Kolatkar PR, Marlor CW, Greve JM, Rossmann MG (Apr 1998). "The structure of the two amino-terminal domains of human ICAM-1 suggests how it functions as a rhinovirus receptor and as an LFA-1 integrin ligand". Proceedings of the National Academy of Sciences of the United States of America 95 (8): 4140–5. doi:10.1073/pnas.95.8.4140. PMC 22455. PMID 9539703.

4.       Polin RS, Bavbek M, Shaffrey ME, Billups K, Bogaev CA, Kassell NF, Lee KS (Oct 1998). "Detection of soluble E-selectin, ICAM-1, VCAM-1, and L-selectin in the cerebrospinal fluid of patients after subarachnoid hemorrhage". Journal of Neurosurgery 89 (4): 559–67. doi:10.3171/jns.1998.89.4.0559. PMID 9761049.