• PN: B213335

COMPONENTS

45x Ab-conjugated beads (S4P4 - Mouse sE-selectin Ab-bead). PN: A213203A. One vial containing 100 µL of anti-mouse sE-selectin conjugated to AimPlex Bead S4P4.

25x Biotin-detection Ab (Mouse sE-selectin Biotin-dAb). PN: B A213203B. One vial containing 100 µL of biotinylated anti-mouse sE-selectin.

Lyophilized Standard Mix - Mouse Group 5 Panel B, 10-Plex. PN: MG5010B. One vial containing lyophilized recombinant sCD27, sCD105, sIFNγR1, sTNFR1, sTNFRII, sTWEAK-R, sVEGFR1, sVEGFR2, sIL-1R1, and sRAGE.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Mouse IFNγR1/sIFNγRa/sCD119. Can be multiplexed with other analytes in Mouse Group 5.  To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 20 pg/mL

Quantitation range:

LLOQ: < 50 pg/mL

ULOQ: > 10,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 5: Negligible

Sample volume: 15 µL/test

Description:

Interferon gamma receptor 1 (IFNGR1) also known as CD119, encodes the ligand-binding chain (alpha) of the heterodimeric gamma interferon receptor, which is found on macrophages. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection. Diseases associated with IFNGR1 include immunodeficiency 27a, mycobacteriosis and H. pylori infection.

References:

1.      Novick D, Orchansky P, Revel M, Rubinstein M (Jun 1987). "The human interferon-gamma receptor. Purification, characterization, and preparation of antibodies". The Journal of Biological Chemistry 262 (18): 8483–7. PMID 2954953.

2.      Aguet M, Dembić Z, Merlin G (Oct 1988). "Molecular cloning and expression of the human interferon-gamma receptor". Cell 55 (2): 273–80. doi:10.1016/0092-8674(88)90050-5. PMID 2971451.

3.      Thiel DJ, le Du MH, Walter RL, D'Arcy A, Chène C, Fountoulakis M, Garotta G, Winkler FK, Ealick SE (Sep 2000). "Observation of an unexpected third receptor molecule in the crystal structure of human interferon-gamma receptor complex". Structure 8 (9): 927–36. doi:10.1016/S0969-2126(00)00184-2. PMID 10986460.