• PN: A211113

COMPONENTS

45x Ab-conjugated beads (S4P6 - mouse IL-1α Ab-bead). PN: A211113A. One vial containing 100 µL of anti-mouse IL-1α conjugated to AimPlex Bead S4P6.

25x Biotin-detection Ab (Mouse IL-1α Biotin-dAb). PN: A211113B. One vial containing 100 µL of biotinylated anti-mouse IL-1α.

Lyophilized Standard Mix - Mouse Group 1 Panel B, 9-Plex. PN: MG10009. One vial containing lyophilized recombinant mouse Eotaxin, IL-1α, KC, IL-12p70, IP-10, MIG, MCP-1, MCP-3, and RANTES.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Mouse IL-1α/IL1A/IL-1F1. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 2 pg/mL

Quantitation range:

LLOQ: <10 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 1: Negligible

Sample volume: 15 µL/test

Description:

Interleukin-1 alpha (IL-1α or IL1A), also known as hematopoietin-1, is a protein of the interleukin 1 family. In general, Interleukin 1 is responsible for the production of inflammation, as well as the promotion of fever and sepsis. IL-1α inhibitors are being developed to interrupt those processes and treat diseases. IL-1 alpha is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, hematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. It is on the pathway that activates tumor necrosis factor-alpha. Shortly after an onset of an infection into organism, IL-1α activates a set of immune system response processes. In particular, IL-1α:  stimulates fibroblasts proliferation, induces synthesis of proteases, subsequent muscle proteolysis, release of all types of amino acids in blood and stimulates acute-phase proteins synthesis,  changes the metallic ion content of blood plasma by increasing copper and decreasing zinc and iron concentration in blood,    increases blood neutrophils, activates lymphocyte proliferation and induces fever.

References:

1.      Nicklin MJ, Weith A, Duff GW (Jan 1994). "A physical map of the region encompassing the human interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist genes". Genomics 19 (2): 382–4. doi:10.1006/geno.1994.1076. PMID 8188271.

2.      March CJ, Mosley B, Larsen A, Cerretti DP, Braedt G, Price V, Gillis S, Henney CS, Kronheim SR, Grabstein K (Aug 1985). "Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs". Nature 315 (6021): 641–7. doi:10.1038/315641a0. PMID 2989698.

3.      Bankers-Fulbright JL, Kalli KR, McKean DJ (1996). "Interleukin-1 signal transduction". Life Sciences 59 (2): 61–83. doi:10.1016/0024-3205(96)00135-X. PMID 8699924.

4.      Dinarello CA (Jun 1997). "Induction of interleukin-1 and interleukin-1 receptor antagonist". Seminars in Oncology 24 (3 Suppl 9): S9–81–S9–93. PMID 9208877.