96 Tests PN: A211116

32 Tests PN: A211116T

Components:

45x Ab-conjugated beads (S5P4 - mouse IL-1β Ab-bead). 

  • 96 Tests PN: A211116A. One vial containing 100 µL of anti-mouse IL-1β conjugated to AimPlex Bead S5P4.

  • 32 Tests PN: A211116TA. One vial containing 35 µL of anti-mouse IL-1β conjugated to AimPlex Bead S5P4.

25x Biotin-detection Ab (Mouse IL-1β Biotin-dAb). 

  • 96 Tests PN: A211116B. One vial containing 100 µL of biotinylated anti-mouse IL-1β.

  • 32 Tests PN: A211116TB. One vial containing   35 µL of biotinylated anti-mouse IL-1β.

Lyophilized Standard Mix - Group 2 Panel A, 10-Plex. 

  • 96 & 32 Tests PN: MG2009A. One vial containing lyophilized recombinant mouse IL-1β, IL-3, IL-5, IL-7, IL-12/IL-23p40, IL-13, IL-15, IL-17F, IL-21, and IL-22.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.


Application: Optimal antibody pair and antigen standard for assaying human Mouse IL-1β/IL-1F2. Can be multiplexed with other analytes in Mouse Group 2. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (96 Tests PN: P200201, 32 Tests PN: P200201T) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 2 pg/mL

  • Quantitation range:

  • LLOQ: < 5 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Mouse Group 2: Negligible

  • Sample volume: 15 µL/test

Description:

Interleukin-1 beta (IL-1β or IL1B) also known as '"leukocytic pyrogen"', "'leukocytic endogenous mediator'", "'mononuclear cell factor'", "'lymphocyte activating factor'" and other names, is a cytokine protein. IL-1β is a member of the interleukin 1 family of cytokines and is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Increased production of IL-1B causes a number of different auto-inflammatory syndromes, most notably the monogenic conditions referred to as CAPS, due to mutations in the inflammasome receptor NLRP3 which triggers processing of IL-1B.

References:

  1. Auron PE, Webb AC, Rosenwasser LJ, Mucci SF, Rich A, Wolff SM, Dinarello CA (1984). "Nucleotide sequence of human monocyte interleukin 1 precursor cDNA". Proc. Natl. Acad. Sci. U.S.A. 81 (24): 7907–11. doi:10.1073/pnas.81.24.7907. PMC 392262. PMID 6083565.

  2. "Catabolin" is the name given by Jeremy Saklatvala for IL-1 alpha. March CJ, Mosley B, Larsen A, Cerretti DP, Braedt G, Price V, Gillis S, Henney CS, Kronheim SR, Grabstein K (1985). "Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs". Nature 315 (6021): 641–7. doi:10.1038/315641a0. PMID 2989698.

  3. Clark BD, Collins KL, Gandy MS, Webb AC, Auron PE (1986). "Genomic sequence for human prointerleukin 1 beta: possible evolution from a reverse transcribed prointerleukin 1 alpha gene". Nucleic Acids Res 14 (20): 7897–1914. doi:10.1093/nar/14.20.7897. PMID 3490654Masters SL, Simon A, Aksentijevich I, Kastner DL (2009). "Horror autoinflammaticus: the molecular pathophysiology of autoinflammatory disease (*)". Annu. Rev. Immunol. 27: 621–68. doi:10.1146/annurev.immunol.25.022106.141627. PMC 2996236. PMID 19302049.