- PN: A211140
45x Ab-conjugated beads (S4P2 - mouse IL-2 Ab-bead). PN: A211140A. One vial containing 100 µL of anti-mouse IL-2 conjugated to AimPlex Bead S4P2.
25x Biotin-detection Ab (Mouse IL-2 Biotin-dAb). PN: A211140B. One vial containing 100 µL of biotinylated anti-mouse IL-2.
Lyophilized Standard Mix - Mouse Group 1 Panel A, 7-Plex. PN: MG10007. One vial containing lyophilized recombinant mouse IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, and TNFα. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
STORAGE: 2-8 C in the dark.
IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
APPLICATION: Optimal antibody pair and antigen standard for assaying human Mouse IL-2. Can be multiplexed with other analytes in Mouse Group 1. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 2 pg/mL
LLOQ: < 5 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Human Group 1: Negligible
Sample volume: 15 µL/test
Interleukin 2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a protein that regulates the activities of leukocytes. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 has roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells. In the thymus, where T cells mature, it prevents autoimmune diseases by promoting the differentiation of certain immature T cells into regulatory T cells, which suppress other T cells that are otherwise primed to attack normal healthy cells in the body. IL-2 also promotes the differentiation of T cells into effector T cells and into memory T cells when the initial T cell is also stimulated by an antigen, thus helping the body fight off infections. Its expression and secretion is tightly regulated and functions as part of both transient positive and negative feedback loops in mounting immune responses and tamping them down. Through its role in the development of T cell immunologic memory, which depends upon the expansion of the number and function of antigen-selected T cell clones, it also has a key role in enduring cell-mediated immunity.
- Liao W, Lin JX, Leonard WJ (Oct 2011). "IL-2 family cytokines: new insights into the complex roles of IL-2 as a broad regulator of T helper cell differentiation". Current Opinion in Immunology 23 (5): 598–604. doi:10.1016/j.coi.2011.08.003. PMC 3405730. PMID 21889323.
- Gaffen SL, Liu KD (Nov 2004). "Overview of interleukin-2 function, production and clinical applications". Cytokine 28 (3): 109–23. doi:10.1016/j.cyto.2004.06.010. PMID 15473953.
- Malek TR, Castro I (Aug 2010). "Interleukin-2 receptor signaling: at the interface between tolerance and immunity". Immunity 33 (2): 153–65. doi:10.1016/j.immuni.2010.08.004. PMC 2946796. PMID 20732639.
- Reich A, Szepietowski JC (2007). "Mediators of pruritus in psoriasis". Mediators of Inflammation 2007: 64727. doi:10.1155/2007/64727d. PMC 2221678. PMID 18288273.