- PN: A211164
45x Ab-conjugated beads (S5P5 - Mouse IL-5 Ab-bead). PN: A211164A. One vial containing 100 µL of anti-mouse IL-5 conjugated to AimPlex Bead S5P5.
25x Biotin-detection Ab (Mouse IL-5 Biotin-dAb). PN: A211164B. One vial containing 100 µL of biotinylated anti-mouse IL-5.
Lyophilized Standard Mix - Group 2 Panel A, 10-Plex. PN: MG2009A. One vial containing lyophilized recombinant mouse IL-1β, IL-3, IL-5, IL-7, IL-12/IL-23p40, IL-13, IL-15, IL-17F, IL-21, and IL-22. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
STORAGE: 2-8 C in the dark.
IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
APPLICATION: Optimal antibody pair and antigen standard for assaying human Mouse IL-5. Can be multiplexed with other analytes in Mouse Group 2. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 5 pg/mL
LLOQ: < 10 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Human Group 2: Negligible
Sample volume: 15 µL/test
Interleukin 5 or IL-5 is an interleukin produced by T helper-2 cells and mast cells. Through binding to the IL-5 receptor, IL-5 stimulates B cell growth and increases immunoglobulin secretion. It is also a key mediator in eosinophil activation. Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma, wherein a large increase in the number of circulating, airway tissue, and induced sputum eosinophils have been observed. Given the high concordance of eosinophils and, in particular, allergic asthma pathology, it has been widely speculated that eosinophils have an important role in the pathology of this disease.
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2. Lee JS, Campbell HD, Kozak CA, Young IG (March 1989). "The IL-4 and IL-5 genes are closely linked and are part of a cytokine gene cluster on mouse chromosome 11". Somat. Cell Mol. Genet. 15 (2): 143–52. doi:10.1007/BF01535075. PMID 2784591.
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