45x Ab-conjugated beads (S5P2 - Mouse Leptin Ab-bead). PN: B211254A. One vial containing 100 µL of anti-mouse Leptin conjugated to AimPlex Bead S5P2.
25x Biotin-detection Ab (Mouse Leptin Biotin-dAb). PN: B211254B. One vial containing 100 µL of biotinylated anti-mouse Leptin.
Lyophilized Standard Mix - Mouse Group 3 Panel A, 8-Plex. PN: MG3008A. One vial containing lyophilized recombinant mouse BTC, IL-1RA, IL-9, IL-23p19, IL-25, Leptin, LIF and VEGF. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
STORAGE: 2-8 C in the dark.
IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
APPLICATION: Optimal antibody pair and antigen standard for assaying human Mouse Leptin/LEP. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 2 pg/mL
LLOQ: < 5 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Mouse Group 3: Negligible
Sample volume: 15 µL/test
Leptin (LEP), a hormone that is secreted by white adipocytes, plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. Leptin also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in the gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene that encodes Leptin has also been linked to type 2 diabetes mellitus development.
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