• PN: B212282

COMPONENTS

45x Ab-conjugated beads (S4P9 - Mouse MCP-2 Ab-bead). PN: B212282A. One vial containing 100 µL of anti-mouse MCP-2 conjugated to AimPlex Bead S4P9.

25x Biotin-detection Ab (Mouse MCP-2 Biotin-dAb). PN: B212282B. One vial containing 100 µL of biotinylated anti-mouse MCP-2.

Lyophilized Standard Mix - Mouse MCP-2. PN: MMCP2AG. One vial containing lyophilized recombinant mouse MCP-2.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Mouse CCL8/MCP-2. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 10 pg/mL

Quantitation range:

LLOQ: < 20 pg/mL

ULOQ: > 2,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Sample volume: 15 µL/test

Description:

Chemokine (C-C motif) ligand 8 (CCL8), also known as monocyte chemoattractant protein 2 (MCP2), is a small cytokine belonging to the CC chemokine family. CCL8 is chemotactic for and activates many different immune cells, including mast cells, eosinophils and basophils that are implicated in allergic responses, monocytes, T cells, and NK cells that are involved in the inflammatory response. CCL8 elicits its effects by binding to several different chemokine receptors which includes CCR1, CCR2B and CCR5.  CCL8 is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes.  CCL8 is a potent inhibitor of HIV1 due to its high-affinity binding to the receptor CCR5, one of the major co-receptors for HIV1. Diseases associated with CCL8 include Purulent Labyrinthitis and Primary Syphilis.

References:

1.      Van Coillie E, Fiten P, Nomiyama H, Sakaki Y, Miura R, Yoshie O, Van Damme J, Opdenakker G (March 1997). "The human MCP-2 gene (SCYA8): cloning, sequence analysis, tissue expression, and assignment to the CC chemokine gene contig on chromosome 17q11.2". Genomics. 40 (2): 323–31. doi:10.1006/geno.1996.4594. PMID 9119400. 

2.      Van Damme J, Proost P, Lenaerts JP, Opdenakker G (July 1992). "Structural and functional identification of two human, tumor-derived monocyte chemotactic proteins (MCP-2 and MCP-3) belonging to the chemokine family". J. Exp. Med. 176 (1): 59–65. doi:10.1084/jem.176.1.59. PMC 2119277. PMID 1613466. 

3.      Proost P, Wuyts A, Van Damme J (January 1996). "Human monocyte chemotactic proteins-2 and -3: structural and functional comparison with MCP-1". J. Leukoc. Biol. 59 (1): 67–74. PMID 8558070. 

4.      Gong W, Howard OM, Turpin JA, Grimm MC, Ueda H, Gray PW, Raport CJ, Oppenheim JJ, Wang JM (February 1998). "Monocyte chemotactic protein-2 activates CCR5 and blocks CD4/CCR5-mediated HIV-1 entry/replication". J. Biol. Chem. 273 (8): 4289–92. doi:10.1074/jbc.273.8.4289. PMID 9468473. 

5.      Blaszczyk J, Coillie EV, Proost P, Damme JV, Opdenakker G, Bujacz GD, Wang JM, Ji X (November 2000). "Complete crystal structure of monocyte chemotactic protein-2, a CC chemokine that interacts with multiple receptors". Biochemistry. 39 (46): 14075–81. doi:10.1021/bi0009340. PMID 11087354.