96 Tests PN: B211260
45x Ab-conjugated beads (S5P9 - Mouse M-CSF Ab-bead). PN: B211260A. One vial containing 100 µL of anti-mouse M-CSF conjugated to AimPlex Bead S5P9.
25x Biotin-detection Ab (Mouse M-CSF Biotin-dAb). PN: B211260B. One vial containing 100 µL of biotinylated anti-mouse M-CSF.
Lyophilized Standard Mix - Mouse Group 2 Panel B, 8-Plex. PN: MG20008. One vial containing lyophilized recombinant mouse G-CSF, GM-CSF, IL-28A/B, M-CSF, MIP-1α, MIP-1β, OSM, and TSLP. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
Application: Optimal antibody pair and antigen standard for assaying human Mouse M-CSF/CSF-1. Can be multiplexed with other analytes in Mouse Group 2. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
Storage: 2-8 C in the dark.
Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 5 pgmL
LLOQ: < 10 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Mouse Group 2: Negligible
Sample volume: 15 µL/test
Colony stimulating factor 1 (CSF1), also known as macrophage colony-stimulating factor (M-CSF), is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. Eukaryotic cells also produce M-CSF in order to combat intercellular viral infection. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. M-CSF released by osteoblasts (as a result of endocrine stimulation by parathyroid hormone) exerts paracrine effects on osteoclasts. Additionally, high levels of CSF-1 expression are observed in the endometrial epithelium of the pregnant uterus as well as high levels of its receptor CSF1R in the placental trophoblast. Locally produced M-CSF in the vessel wall contributes to the development and progression of atherosclerosis. M-CSF has been described to play a role in renal pathology including acute kidney injury (AKI) and chronic renal failure (CRF).
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